Effects of ozone on influenza virus-induced airway hyperreactivity in rats.
- Author:
Hye Kyung LEE
1
Author Information
1. Department of Pharmacology, Chonbuk National University Dental School Chonju, Korea.
- Publication Type:Original Article
- Keywords:
Ozone;
influenza virus;
airway hyperreactivity;
acetylcholine;
air pollutant
- MeSH:
Acetylcholine;
Airway Resistance;
Animals;
Asthma;
Influenza, Human*;
Infusions, Intravenous;
Mechanics;
Orthomyxoviridae;
Ozone*;
Rats*
- From:Journal of Asthma, Allergy and Clinical Immunology
2000;20(5):749-754
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Ozone is one of the most ubiquitous oxidant in urban air and may exacerbate respiratory diseases such as asthma. OBJECTIVE: We examined whether ozone can influence airway hyperresponsiveness induced by influenza virus-infection. SUBJECT AND METHOD: Rats were exposed to ozone, acutely to 1.0ppm and 0.25ppm for 23.5hrs. At 2hrs post cessation of ozone exposure, animals were infected with rat-adapted influenza virus intra-nasally. On day 3 post-virus inoculation, pulmonary mechanics were measured during an intravenous challenge with acetylcholine < (ACh), 100-140mg/ml in saline>. Cumulative doseresponse functions were obtained by doubling the rate of the intravenous infusion every 2min. RESULTS: ACh increased airway resistance in a dose-dependent manner. Neither of the concentrations of ozone altered the airway responses to ACh. Animals infected with virus developed hyperresponsiveness to ACh as was expected. Pre-exposure to 1.0ppm ozone for 23.5hrs aug- mented the virus-induced airway hyperresponsiveness, while 0.25ppm did not. CONCLUSION: Acute exposure to ozone can synergistically act on virus-induced airway hyperreactivity.
