Crotonis Fructus Extract Inhibits 12-O-Tetradecanoylphorbol-13-Acetate-Induced Expression of Matrix Metalloproteinase-9 via the Activator Protein-1 Pathway in MCF-7 Cells.
10.4048/jbc.2017.20.3.234
- Author:
Hyun Kyung SONG
1
;
Guem San LEE
;
Sueng Hyuk PARK
;
Eun Mi NOH
;
Jeong Mi KIM
;
Do Gon RYU
;
Sung Hoo JUNG
;
Hyun Jo YOUN
;
Young Rae LEE
;
Kang Beom KWON
Author Information
1. Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Korea. desson@wku.ac.kr
- Publication Type:Original Article
- Keywords:
Crotonis fructus;
Matrix metalloproteinase 9;
MCF-7 cells;
Neoplasm invasiveness;
Transcription factor AP-1
- MeSH:
Asia;
Blotting, Western;
Breast Neoplasms;
Cell Survival;
Croton;
DNA;
Ethanol;
Fruit;
In Vitro Techniques;
Matrix Metalloproteinase 9*;
MCF-7 Cells*;
Neoplasm Invasiveness;
Protein Kinase C;
Real-Time Polymerase Chain Reaction;
Transcription Factor AP-1*
- From:Journal of Breast Cancer
2017;20(3):234-239
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Metastatic cancers spread from the primary site of origin to other parts of the body. Matrix metalloproteinase-9 (MMP-9) is essential in metastatic cancers owing to its major role in cancer cell invasion. Crotonis fructus (CF), the mature fruits of Croton tiglium L., have been used for the treatment of gastrointestinal disturbance in Asia. In this study, the effect of the ethanol extract of CF (CFE) on MMP-9 activity and the invasion of 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 cells was examined. METHODS: The cell viability was evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The expression of MMP-9 was examined by Western blotting, zymography, and real-time polymerase chain reaction. An electrophoretic mobility gel shift assay was performed to detect activator protein-1 (AP-1) DNA binding activity and cell invasiveness was measured by an in vitro Matrigel invasion assay. RESULTS: CFE significantly suppressed MMP-9 expression and activation in a dose-dependent manner. Furthermore, CFE attenuated the TPA-induced activation of AP-1. CONCLUSION: The results indicated that the inhibitory effects of CFE against TPA-induced MMP-9 expression and MCF-7 cell invasion were dependent on the protein kinase C δ/p38/c-Jun N-terminal kinase/AP-1 pathway. Therefore, CFE could restrict breast cancer invasiveness owing to its ability to inhibit MMP-9 activity.
