SOCS1 Regulates the Immunomodulatory Roles of MSCs on B Cells
- Author:
Lei ZHANG
1
;
Yan-Nv QU
;
He-Yang ZHANG
;
Zhen-Yang WU
;
Zhong-Li LI
;
Wan-Bei GUO
;
Qi-Ben WANG
;
Nan-Zhu FANG
;
Xiao-Xia JIANG
Author Information
- Publication Type:ORIGINAL ARTICLE
- From:International Journal of Stem Cells 2020;13(2):237-245
- CountryRepublic of Korea
- Language:0
-
Abstract:
Background and Objectives:The effective use of MSCs for the treatment of some B cell-mediated immune diseases is quite limited. The main reason is that the immunomodulatory effects of mesenchymal stem cells (MSCs) on B cells are unclear, and their underlying mechanisms have not been fully explored.
Methods:and Results: By co-culturing B cells with MSCs without (MSC/CTLsh) or with suppressor of cytokine signaling 1 (SOCS1) knockdown (MSC/SOCS1sh), we found that MSCs inhibited B cell proliferation, activation and terminal differentiation. Remarkably, the highest inhibition of B cell proliferation was observed in MSC/SOCS1sh co-culture. Besides, MSC/SOCS1sh reversed the inhibitory effect of MSCs in the last stage of B cell differentiation. However, MSC/SOCS1sh had no effect on inhibiting B cell activation by MSCs. We also showed that IgA+ B cell production was significantly higher in MSC/SOCS1sh than in MSC/CTLsh, although no difference was observed when both MSCs co-cultures were compared to isolated B cells. In addition, MSCs increased PGE2 production after TNF-α/IFN-γ stimulation, with the highest increase observed in MSC/SOCS1sh co-culture.
Conclusions:Our results highlighted the role of SOCS1 as an important new mediator in the regulation of B cell function by MSCs. Therefore, these data may help to develop new treatments for B cell-mediated immune diseases.
