Overexpression of p21 Has Inhibitory Effect on Human Hematopoiesis by Blocking Generation of CD43＋ Cells via Cell-Cycle Regulation
- Author:
Jiahui ZENG
1
;
Huifang ZHANG
;
Yuanling LIU
;
Wencui SUN
;
Danying YI
;
Lijiao ZHU
;
Yonggang ZHANG
;
Xu PAN
;
Yijing CHEN
;
Ya ZHOU
;
Guohui BIAN
;
Mowen LAI
;
Qiongxiu ZHOU
;
Jiaxin LIU
;
Bo CHEN
;
Feng MA
Author Information
- Publication Type:ORIGINAL ARTICLE
- From:International Journal of Stem Cells 2020;13(2):202-211
- CountryRepublic of Korea
- Language:0
-
Abstract:
Background and Objectives:p21, an important member of the Cip/Kip family, is involved in inhibitory effects of RUNX1b overexpression during the early stage of human hematopoiesis.
Methods:and Results: We established a human embryonic stem cell (hESC) line with inducible expression of p21 (p21/hESCs). Overexpression of p21 did not influence either mesoderm induction or emergence of CD34＋ cells, but it significantly decreased the production of CD43＋ cells and changed the expression profile of hematopoiesis-related factors, leading to the negative effects of p21 on hematopoiesis.
Conclusions:In RUNX1b/hESC co-cultures when RUNX1b was induced from D0, perturbation of the cell cycle caused by upregulation of p21 probably prevented the appearance of CD43＋ cells, but not CD34＋ cells. The mechanisms via which CD34＋ cells are blocked by RUNX1b overexpression remain to be elucidated.
