Adipose Tissue-Derived Stem Cells from Type 2 Diabetics Reveal Conservative Alterations in Multidimensional Characteristics
- Author:
Le WANG
1
;
Leisheng ZHANG
;
Xue LIANG
;
Jiaqi ZOU
;
Na LIU
;
Tengli LIU
;
Guanqiao WANG
;
Xuejie DING
;
Yaojuan LIU
;
Boya ZHANG
;
Rui LIANG
;
Shusen WANG
Author Information
- Publication Type:ORIGINAL ARTICLE
- From:International Journal of Stem Cells 2020;13(2):268-278
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background and Objectives:Adipose tissue-derived mesenchymal stem cells (ASCs) are recognized as an advantaged source for the prevention and treatment of diverse diseases including type 2 diabetes mellitus (T2DM). However, alterations in characteristics of ASCs from the aforementioned T2DM patients are still obscure, which also hinder the rigorous and systematic illumination of progression and pathogenesis.
Methods:and Results: In this study, we originally isolated peripancreatic adipose tissue-derived mesenchymal stem cells from both human type 2 diabetic and non-diabetic donors (T2DM-ASCs, ND-ASCs) with the parental consent, respectively. We noticed that T2DM-ASCs exhibited indistinguishable immunophenotype, cell vitality, chondrogenic differentiation and stemness as ND-ASCs. Simultaneously, there’s merely alterations in migration and immunoregulatory capacities in T2DM-ASCs. However, differing from ND-ASCs, T2DM-ASCs exhibited deficiency in adipogenic and osteogenic differentiation, and in particular, the delayed cell cycle and different cytokine expression spectrum.
Conclusions:The conservative alterations of T2DM-ASCs in multifaceted characteristics indicated the possibility of autologous application of ASCs for cell-based T2DM treatment in the future.
