Calcitonin Gene-related Peptide Immunoreactivity in the Muscle Layer of Small Intestine; Its Action on Interstitial Cell.
10.11637/kjpa.2004.17.3.207
- Author:
Sang Pil YOON
1
;
Jae Yeoul JUN
;
Young LIM
;
In Jeong KIM
;
Joo Young KIM
;
Jang Man KIM
;
In Youb CHANG
Author Information
1. Department of Anatomy, College of Medicine, Chosun University, Korea. iyjang@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Calcitonin gene-related peptide(CGRP);
Enteric muscle;
Bowel motility;
Mouse
- MeSH:
Animals;
Calcitonin Gene-Related Peptide*;
Calcitonin*;
Enteric Nervous System;
Ganglion Cysts;
Immunohistochemistry;
Interstitial Cells of Cajal;
Intestine, Small*;
Mice;
Nerve Fibers;
Peripheral Nervous System;
Relaxation
- From:Korean Journal of Physical Anthropology
2004;17(3):207-214
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In addition to the central and the peripheral nervous system, calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) has been identified throughout the enteric nervous system. Several functions of the CGRP in gastrointestinal (G-I) tract has been identified, but the effect of CGRP on G-I motility is unclear. The distribution of calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) in the murine small bowel were studied by using immunohistochemistry, also analyzed functionally by using electrophysiological method. Immunohistochemical studies demonstrated that CGRP-LI is localized in both nerve fibers and myenteric ganglion cells in the whole-mount preparation of murine small intestine. Double labelling with CGRP and c-kit investigated by confocal microscope was shown that CGRP-LI enteric nerve fiber surrounded the c-kit positive interstitial cells of Cajal (ICC). Electrophysiological finding revealed that treatment of CGRP inhibited electrical activity on culture ICC. Our results suggest a CGRP innervation of murine small bowel ICC. The released CGRP from enteric nerve terminals may induce relaxation of small bowel through the inhibition of ICC.
