Effects of magnesium sulfate on VEGF and Caspase-3 of HUVECs (Human umbilical vein endothelial cells).
- Author:
Gui Se Ra LEE
1
;
Sa Jin KIM
;
So Young KIM
;
Young LEE
;
Sang Hi PARK
;
Jong Chul SHIN
;
Young Oak LEW
;
Soo Pyung KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Catholic University of Korea, Seoul, Korea. leegsr@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
HUVECs;
TNF-alpha;
Magnesium sulfate;
VEGF;
Caspase-3
- MeSH:
Caspase 3*;
Enzyme-Linked Immunosorbent Assay;
Human Umbilical Vein Endothelial Cells;
Magnesium Sulfate*;
Magnesium*;
Tumor Necrosis Factor-alpha;
Umbilical Cord;
Umbilical Veins*;
Vascular Endothelial Growth Factor A*
- From:Korean Journal of Perinatology
2004;15(3):262-267
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To determine the effects of magnesium sulfate on Caspase-3 and Vascular endothelial growth factor (VEGF) of human umbilical vein endothelial cells (HUVECs) under presence or absence of Tumor necrosis factor (TNF-alpha). METHODS: HUVECs were isolated from normal term umbilical cords and cultured in several physiolo gically relevant concentrations of magnesium sulfate with or without exposure of TNF-alpha. The concentrations of VEGF and caspase-3 were estimated by colorimetric assay and ELISA assay, respectively. RESULTS: The concentration of VEGF in HUVECs significantly increased in the presence of TNF-alpha compared with in the absence of TNF-alpha. However, the concentration of VEGF did not show significant difference in several concentrations of magnesium sulfate concentrations with addition of TNF-alpha and it showed the lowest concentration under 4 mM and 8 mM of magnesium sulfate concentration without addition of TNF-alpha. The concentration of caspase-3 in HUVECs did not show statistically significant difference with the addition of TNF-alpha and magnesium sulfate. CONCLUSION: TNF-alpha induce HUVECs to stimulate the VEGF, and magnesium sulfate might not have the effects on the expression of VEGF with addition of TNF-alpha However, the concentration for treatment of magnesium sulfate inhibits the expression of VEGF without addition of TNF-alpha. Magnesium sulfate might not have an effect of the expression of caspase-3 with or without addition of TNF-alpha.
