Morphological and Molecular Confirmation of Parvatrema duboisi Metacercariae in the Manila Clam Ruditapes philippinarum from Gochang-gun, Korea
- Author:
Taehee CHANG
1
;
Bong-Kwang JUNG
;
Hyejoo SHIN
;
Sooji HONG
;
Jeonggyu LEE
;
Deok-Gyu KIM
;
Laddawan PATARWUT
;
Woon-Mok SOHN
;
Jong-Yil CHAI
Author Information
- Publication Type:Brief Communication
- From:The Korean Journal of Parasitology 2020;58(1):87-91
- CountryRepublic of Korea
- Language:English
- Abstract: Gymnophallid metacercariae found in the Manila clam Ruditapes philippinarum (‘Banjirak’ in Korean) from Gochang-gun, Jeollabuk-do, Korea were morphologically and molecularly confirmed to be Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974. The metacercariae were morphologically characterized by having a large oral sucker, small ventral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, which were all compatible with P. duboisi. Some of the metacercariae were experimentally fed to mice, and adult flukes were recovered at day 7 post-infection. The morphology of the adult flukes was basically the same as that of the metacercariae except for the presence of uterine eggs; the uterus was filled with up to 40 eggs. The nucleotide sequences (1,193 bp) from ITS regions (ITS1, 5.8S rDNA, and ITS2) of the metacercariae showed 99.7% identity with P. duboisi and 75.7% identity with Gymnophalloides seoi deposited in GenBank. These results confirmed the presence of P. duboisi metacercariae in the Manila clam R. philippinarum in an estuary region of Gochang-gun, Korea.
