Identification of urinary microRNA biomarkers for in vivo gentamicininduced nephrotoxicity models
- Author:
Byung-Suk JEON
1
;
Soo-ho LEE
;
So-Ryeon HWANG
;
Hee YI
;
Ji-Hyun BANG
;
Nga Thi Thu THAM
;
Hyun-Kyoung LEE
;
Gye-Hyeong WOO
;
Hwan-Goo KANG
;
Hyun-Ok KU
Author Information
- Publication Type:Original Article
- From:Journal of Veterinary Science 2020;21(6):e81-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Although previous in vivo studies explored urinary microRNA (miRNA), there is no agreement on nephrotoxicity-specific miRNA biomarkers.
Objectives:In this study, we assessed whether urinary miRNAs could be employed as biomarkers for nephrotoxicity.
Methods:For this, literature-based candidate miRNAs were identified by reviewing the previous studies. Female Sprague-Dawley rats received subcutaneous injections of a single dose or repeated doses (3 consecutive days) of gentamicin (GEN; 137 or 412 mg/kg). The expression of miRNAs was analyzed by real-time reverse transcription-polymerase chain reaction in 16 h pooled urine from GEN-treated rats.
Results:GEN-induced acute kidney injury was confirmed by the presence of tubular necrosis.We identified let-7g-5p, miR-21-3p, 26b-3p, 192-5p, and 378a-3p significantly upregulated in the urine of GEN-treated rats with the appearance of the necrosis in proximal tubules.Specifically, miR-26-3p, 192-5p, and 378a-3p with highly expressed levels in urine of rats with GEN-induced acute tubular injury were considered to have sensitivities comparable to clinical biomarkers, such as blood urea nitrogen, serum creatinine, and urinary kidney injury molecule protein.
Conclusions:These results indicated the potential involvement of urinary miRNAs in chemical-induced nephrotoxicity, suggesting that certain miRNAs could serve as biomarkers for acute nephrotoxicity.
