NUP210 and MicroRNA-22 Modulate Fasto Elicit HeLa Cell Cycle Arrest
10.3349/ymj.2020.61.5.371
- Author:
Qiao GU
1
;
Wenjie HOU
;
Huan LIU
;
Lijuan SHI
;
Zonghao ZHU
;
Wenfeng YE
;
Xiaoyuan NI
Author Information
1. Department of Gynecology and Obstetrics, The Third Affiliated Hospital of Soochow University, Changzhou, China
- Publication Type:Original Article
- From:Yonsei Medical Journal
2020;61(5):371-381
- CountryRepublic of Korea
-
Abstract:
Purpose:Cervical cancer is one of the most fatal diseases among women in under-developed countries. To improve cervical cancertreatment, discovery of new targets is needed. In this study, we investigated the expression of NUP210, miR-22, and Fas in cervicalcancer tissues and their functions in cell cycle regulation.
Materials and Methods:We detected and compared the expression levels of NUP210, miR-22, and Fas in cervical cancer tissueswith paired normal tissues using immunohistochemistry, Western blot, and real-time quantitative polymerase chain reaction.NUP210 was knocked down in HeLa cells via lentivirus, followed by cell cycle and proliferation analysis. Using a luciferase reporterassay, we explored the link between miR-22 and NUP210. We overexpressed miR-22 in HeLa cells and analyzed cell cycle and proliferationfunction. We then overexpressed miR-22 in NUP210 knockdown cells to explore the connection between Fas and miR-22-NUP210 signaling.
Results:We found that NUP210 was overexpressed in cervical cancer patients. Knocking down NUP210 restored cell apoptosisand proliferation. We confirmed miR-22 as a regulator of NUP210 and verified that miR-22 was inhibited in cervical cancer development.We also found that restoring miR-22 expression could induce cell apoptosis. Finally, we found that miR-22-regulated expressionof NUP210 could alter Fas expression and, in turn, elicit cell cycle arrest and proliferation.
Conclusion:miR-22 in cervical cancer is downregulated, resulting in NUP210 overexpression and inhibition of Fas-induced cellapoptosis.
