Loss of heterozygosity on chromosome 10, 13q(Rb), 17p, and p53 gene mutations in human brain gliomas.
10.3346/jkms.1995.10.6.442
- Author:
Seung Hoon LEE
1
;
Jong Hyun KIM
;
Chang Hun RHEE
;
Young Soon KANG
;
Je Ho LEE
;
Seok Il HONG
;
Kil Soo CHOI
Author Information
1. Department of Neurosurgery, Korea Cancer Center Hospital, Seoul, Korea.
- Publication Type:Original Article ; Comparative Study
- Keywords:
Loss of heterosygosity (LOH);
Glioma;
Tumor suppressor gene;
p53 gene;
Rb gene
- MeSH:
Astrocytoma/genetics/pathology;
Base Sequence;
Brain Neoplasms/*genetics/pathology;
*Chromosomes, Human, Pair 10;
*Chromosomes, Human, Pair 13;
*Chromosomes, Human, Pair 17;
Comparative Study;
*Gene Deletion;
*Genes, Retinoblastoma;
*Genes, p53;
Glioma/*genetics/pathology;
Heterozygote;
Human;
Molecular Sequence Data;
*Mutation;
Oligodendroglioma/genetics/pathology;
Polymorphism, Restriction Fragment Length;
Polymorphism, Single-Stranded Conformational
- From:Journal of Korean Medical Science
1995;10(6):442-448
- CountryRepublic of Korea
- Language:English
-
Abstract:
Using the methods of restriction fragment length polymorphism (RFLP) and single strand conformation polymorphism (SSCP) analyses, we have examined 33 cases of human gliomas with various malignant grades to detect the deletions of putative tumor suppressor gene loci, chromosome 10, 13q(retinoblastoma gene, Rb), 17p, and p53 mutation. We observed loss of heterozygosity (LOH) at loci on chromosome 10 (36%), 13q(Rb) (54%), and 17p(50%) in malignant gliomas. There, however was no allelic loss on chromosome 10 and 17p in low-grade gliomas. Rb gene deletions were seen in low-grade gliomas, including oligodendroglioma and ependymoma. This finding suggests that Rb inactivation may be an early genetic event in the development and progression of gliomas. We correlated the results of LOH on chromosome 17p and p53 mutation. Among the 8 cases which showed LOH on chromosome 17p, only three cases (38%) revealed p53 mutations. Low incidence of p53 mutations in cases with chromosome 17p deletions suggests that some other tumor suppressor genes may be located on chromosome 17p.
