Expression of Cellular Receptors in the Ischemic Hemisphere of Mice with Increased Glucose Uptake
- Author:
Jin Soo LEE
1
;
Ji Man HONG
;
Bok Seon YOON
;
Keoung Sun SON
;
Kyung Eon LEE
;
Doo Soon IM
;
Bok-Nam PARK
;
Young-Sil AN
;
Dong Hoon HWANG
;
Chan Bae PARK
;
Byung Gon KIM
;
Eun-hye JOE
Author Information
- Publication Type:Original Article
- From:Experimental Neurobiology 2020;29(1):70-79
- CountryRepublic of Korea
- Language:0
- Abstract: Many previous studies have shown reduced glucose uptake in the ischemic brain. In contrast, in a permanent unilateral common carotid artery occlusion (UCCAO) mouse model, our pilot experiments using 18F-fluorodeoxyglucose positron emission tomography (FDG PET) revealed that a subset of mice exhibited conspicuously high uptake of glucose in the ipsilateral hemisphere at 1 week post-occlusion (asymmetric group), whereas other mice showed symmetric uptake in both hemispheres (symmetric group). Thus, we aimed to understand the discrepancy between the two groups. Cerebral blood flow and histological/metabolic changes were analyzed using laser Doppler flowmetry and immunohistochemistry/Western blotting, respectively. Contrary to the increased glucose uptake observed in the ischemic cerebral hemisphere on FDG PET (p<0.001), cerebral blood flow tended to be lower in the asymmetric group than in the symmetric group (right to left ratio [%], 36.4±21.8 vs. 58.0±24.8, p=0.059). Neuronal death was observed only in the ischemic hemisphere of the asymmetric group. In contrast, astrocytes were more activated in the asymmetric group than in the symmetric group (p<0.05). Glucose transporter-1, and monocarboxylate transporter-1 were also upregulated in the asymmetric group, compared with the symmetric group (p<0.05, respectively). These results suggest that the increased FDG uptake was associated with relatively severe ischemia, and glucose transporter-1 upregulation and astrocyte activation. Glucose metabolism may thus be a compensatory mechanism in the moderately severe ischemic brain.
