Allergen-Dependent Differences in ILC2s Frequencies in Patients With Allergic Rhinitis.
10.4168/aair.2016.8.3.216
- Author:
Dachuan FAN
1
;
Xiangdong WANG
;
Min WANG
;
Yang WANG
;
Liang ZHANG
;
Ying LI
;
Erzhong FAN
;
Feifei CAO
;
Koen VAN CROMBRUGGEN
;
Luo ZHANG
Author Information
1. Department of Otolaryngology Head and Neck Surgery and Department of Allergy, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China. dr.luozhang@139.com
- Publication Type:In Vitro ; Original Article
- Keywords:
ILC2;
IL-33;
IL-25;
group 2 innate lymphoid cell;
allergic rhinitis
- MeSH:
Allergens;
Artemisia;
Cytokines;
Humans;
Interleukin-13;
Interleukin-2;
Interleukin-5;
Lymphocytes;
Pyroglyphidae;
Rhinitis*
- From:Allergy, Asthma & Immunology Research
2016;8(3):216-222
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Group 2 innate lymphoid cells (ILC2s) are a novel population of lineage-negative cells that induce innate type 2 responses by producing the critical Th2-type cytokines IL-5 and IL-13 in response to IL-25 and IL-33 stimulation. ILC2s accumulation in the peripheral blood of patients with allergic rhinitis (AR) is controversial; the precise role of ILC2s in the immunopathogenesis of AR is still not clear. We investigated the role of ILC2s in phenotypic AR sensitized to distinct allergens. METHODS: Flow cytometric analysis of the peripheral blood of 7 healthy controls (HCs), 9 patients monosensitized to house dust mite (HDM), and 8 patients monosensitized to mugwort was performed to quantify ILC2s frequency. Peripheral blood mononuclear cells (PBMCs) were isolated from HDM-AR and mugwort-AR patients, and Lineage- and Lineage+ cells were separated using a fluorescence-activated cell sorter (FACS). IL-5 and IL-13 levels in the supernatants of PBMCs, and Lineage- and Lineage+ cells stimulated with IL-25 and/or IL-33 combined with IL-2 in vitro were assessed using the Milliplex magnetic bead kit. RESULTS: The percentage of ILC2s was significantly elevated in HDM-AR patients compared to mugwort-AR patients and HCs, while no significant difference was found between mugwort-AR patients and HCs. IL-33+/-IL-25 plus IL-2 induced a significantly greater release of IL-5 and IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. IL-25 plus IL-2 also induced a significantly greater release of IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. Stimulation with IL-33 and/or IL-25 combined with IL-2 also induced a significantly greater IL-5 and IL-13 release from Lineage- cells compared to Lineage+ cells. CONCLUSIONS: AR patients sensitized to HDM or mugwort allergen have distinct phenotypic and functional profiles in ILC2s frequencies. ILC2s mediate major type 2 immunity in the development of HDM-AR and may be a potential therapeutic target.
