Endometrial profilin 1: a key player in embryo-endometrial crosstalk
- Author:
Chang-Jin LEE
1
;
Seon-Hwa HONG
;
Min-Ji YOON
;
Kyung-Ah LEE
;
Jung-Jae KO
;
Hwa Seon KOO
;
Jee Hyun KIM
;
Dong Hee CHOI
;
Hwang KWON
;
Youn-Jung KANG
Author Information
- Publication Type:Original Article
- From:Clinical and Experimental Reproductive Medicine 2020;47(2):114-121
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:Despite extensive research on implantation failure, little is known about the molecular mechanisms underlying the crosstalk between the embryo and the maternal endometrium, which is critical for successful pregnancy. Profilin 1 (PFN1), which is expressed both in the embryo and in the endometrial epithelium, acts as a potent regulator of actin polymerization and the cytoskeletal network. In this study, we identified the specific role of endometrial PFN1 during embryo implantation.
Methods:Morphological alterations depending on the status of PFN1 expression were assessed in PFN1-depleted or control cells grown on Matrigel-coated cover glass. Day-5 mouse embryos were cocultured with Ishikawa cells. Comparisons of the rates of F-actin formation and embryo attachment were performed by measuring the stability of the attached embryo onto PFN1-depleted or control cells.
Results:Depletion of PFN1 in endometrial epithelial cells induced a significant reduction in cell-cell adhesion displaying less formation of colonies and a more circular cell shape. Mouse embryos co-cultured with PFN1-depleted cells failed to form actin cytoskeletal networks, whereas more F-actin formation in the direction of surrounding PFN1-intact endometrial epithelial cells was detected. Furthermore, significantly lower embryo attachment stability was observed in PFN1-depleted cells than in control cells. This may have been due to reduced endometrial receptivity caused by impaired actin cytoskeletal networks associated with PFN1 deficiency.
Conclusion:These observations definitively demonstrate an important role of PFN1 in mediating cell-cell adhesion during the initial stage of embryo implantation and suggest a potential therapeutic target or novel biomarker for patients suffering from implantation failure.
