Evaluation of the Performance of ASTA MicroIDSys, a Novel Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry System, in Identification of Bacterial Clinical Isolates
- Author:
Changseung LIU
1
;
Eunjung LEE
;
Dokyun KIM
;
Seok Hoon JEONG
Author Information
- Publication Type:Original Article
- From:Annals of Clinical Microbiology 2020;23(3):195-208
- CountryRepublic of Korea
-
Abstract:
Methods:A total of 889 non-duplicated clinical isolates were included in this study. The results of ASTA MicroIDSys were compared with those of Bruker Biotyper; 16S rRNA sequencing was performed for the species for which results obtained using the two systems did not match. The sequences of rpoB, hisA, and/or recA for the clinical isolates of Acinetobacter species, Klebsiella species, and Burkholderia cepacia complex were analyzed and used as reference identifications.
Results:The concordance rates for bacterial identification using ASTA MicroIDSys and Bruker Biotyper were 100% at the genus level and 98.3% at the species level for isolates belonging to the order Enterobacterales. Similarly, the concordance rates at the genus and species levels were 98.8% and 91.0% for glucose non-fermenting bacilli, 100% and 100% for gram-positive cocci, and 98.9% and 98.9% for other isolates, respectively. ASTA MicroIDSys was expected to correctly identify 97.9% of the 108,251 isolates identified in our clinical microbiology laboratory over the past 5 years.
Conclusion:ASTA MicroIDSys showed excellent performance in bacterial identification for most of the clinically relevant species. Further extension of the database could improve the identification accuracy of ASTA MicroIDSys.
