Genotoxicity evaluation of Wentilactone A
10.12206/j.issn.1006-0111.202004089
- VernacularTitle:Wentilactone A的遗传毒性评价
- Author:
Wenjing SHI
1
;
Yijun TIAN
1
;
Caiguo HUANG
2
;
Yuping ZHU
1
;
Lang YAN
1
;
Yiwen ZHENG
1
Author Information
1. .
2. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Naval Medical University, Shanghai 200433, China.
- Keywords:
Wentilactone A;
Ames test;
chromosomal aberration test;
micronucleus assay;
genetic toxicity
- From:
Journal of Pharmaceutical Practice
2020;38(6):533-538
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the genetic toxicity of Wentilactone A. Methods The classical genotoxicity test combination (Ames test, in vitro CHO cell chromosome aberration test and mouse bone marrow micronucleus test) was used to detect the genotoxicity of Wentilactone A. Results Ames test suggested that Wentilactone A was not mutagenic against Salmonella typhimurium with or without the metabolic activation system (S9) at five doses of 5 000, 500, 50, 5, and 0.5 μg/dish. CHO cell chromosome aberration test suggested that the CHO cells cultured in 4 h and 24 h did not induce chromosomal aberrations in three dose groups at the final concentration of 23.74, 47.48, 94.96 μg/ml, with and without S9. The mouse bone marrow micronucleus test showed no significant difference in the bone marrow micronucleus induction rate of cells at three doses of 100, 200, and 400 mg/kg treated for 24 h and at dose of 400 mg/kg treated for 48 h compared with the solvent control group (P>0.05). Conclusion These results indicated that Wentilactone A did not exhibit genetic toxicity based on the Ames test, CHO chromosomal aberration test and micronucleus assay. It was suggested that Wentilactone A had no genetic toxicity and potential carcinogenicity.
