Effectiveness of Flaxseed Oil on Peripheral Nerve Regeneration Following Crush Injury of Sciatic Nerve in Rat: Behavioural Analysis and an Electron Microscopic Study

Muhammad Danial Che Ramli; Nur Amira Sumari; Neni Widiasmoro Selamat; Hussin Muhammad; Junedah Sanusi

Return

Effectiveness of Flaxseed Oil on Peripheral Nerve Regeneration Following Crush Injury of Sciatic Nerve in Rat: Behavioural Analysis and an Electron Microscopic Study

Author: Muhammad Danial Che Ramli ¹ ; Nur Amira Sumari ¹ ; Neni Widiasmoro Selamat ¹ ; Hussin Muhammad ² ; Junedah Sanusi ³
Author Information

1. Faculty of Health and Life Sciences, Management and Science University, 40100, Shah Alam, Selangor, Malaysia
2. Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Wilayah Persekutuan Kuala Lumpur, Malaysia
3. Faculty of Medicine, Department of Anatomy, Kuala Lumpur
Publication Type:Journal Article
Keywords: Sciatic nerve, Flaxseed oil, Behavioural analysis, Myelin sheath, Injury
From:Malaysian Journal of Medicine and Health Sciences 2020;16(Supp 1,January):106-111
CountryMalaysia
Language:English
Abstract: Introduction: Peripheral nerve injuries (PNI) are a disabling injury as it often results in motor and sensory deficit with cognitive impairment. Flaxseed oil provides a good source of omega-3 fatty acid and it is believed to be able to protect the damaged nerve cell for successful nerve recovery. This study aimed to investigate a potential neuro-regeneration properties of flaxseed oil in treating the PNI. Methods: A total of 65 rats were separated into 4 groups: Group 1: Normal group (n=5), Group 2: Negative group (n=20), Group 3: Experimental group (n=20) and Group 4: Positive control (n=20), all the group were further divided into 4 groups (post-operative 7, 14, 21, 28 days, n=5 for each days). The functional restoration was assessed by walking track analysis (Sciatic Functional Index analysis-SFI) and toe spreading reflex (grading score). Electron microscope studies were performed on sciatic nerve to evaluate the regenerative process through morphologic and morphometric changes. Results: Oral administration of flaxseed oil (experimental group) at 1000 mg/kg body weight/day showed better recovery compared to negative control value. However, there was no significant difference in SFI and toe spreading reflex between positive (mecobalamin) and experimental group (flaxseed oil). Morphological and morphometrical findings indicated increases in the myelin thickness and myelin sheath layer after administration of flaxseed oil. Conclusion: The flaxseed oil supplementation could enhance the neurorestorative capacities of injured sciatic nerve.
Full text:11.2020my0636.pdf