β-arrestin-2 inhibits autophagy by up-regulating PI3K/Akt signal to alleviate liver ischemia-reperfusion injury in mice
10.3969/j.issn.1674-7445.2020.06.007
- VernacularTitle:β-arrestin-2通过上调PI3K/Akt信号抑制自噬以减轻小鼠肝脏缺血-再灌注损伤
- Author:
Li WANG
1
;
Xiaolong CHEN
;
Huiling LIU
;
Jing ZHOU
;
Yidong YANG
;
Hui LI
;
Haoqi CHEN
;
Daorou CHENG
;
Bin WU
;
Guihua CHEN
;
Genshu WANG
Author Information
1. Department of Hepatic Surgery, Liver Transplantation Center, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
- Publication Type:Research Article
- Keywords:
Liver;
Ischemia-reperfusion injury;
Autophagy;
β-arrestin-2;
Gene knock out;
PI3K/Akt signal;
Immunohistochemistry
- From:
Organ Transplantation
2020;11(6):692-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify whether β-arrestin-2 inhibits autophagy by up-regulating PI3K/Akt signal to protect the liver from ischemia-reperfusion injury (IRI) in mice. Methods Twelve β-arrestin-2 knockout (KO) and twelve wild-type (WT) C57BL/6 mice were randomly divided into the KO+sham group, KO+IRI group, WT+sham group and WT+IRI group, six mice in each group. The mouse models with 70% liver IRI were established or sham operation was performed. Relevant experiments were carried out at 6 h after liver reperfusion or operation. The expression levels of apoptosis signal protein cleaved Caspase-3, proliferation signal protein Ki-67 and the PI3K/Akt signal protein p-Akt were detected by immunohistochemical staining. Results Immunohistochemical staining demonstrated that compared with the corresponding sham group, the positive cell count for cleaved Caspase-3, Ki-67 and p-Akt in liver tissues of mice was significantly increased in the KO+IRI and WT+IRI groups (all P < 0.01). Compared with the WT+IRI group, the positive cell count for cleaved Caspase-3 in liver tissues of mice was significantly increased, whereas the positive cell count forKi-67 and p-Akt was significantly decreased in the KO+IRI group (both P < 0.05). Conclusions β-arrestin-2 can mitigate the liver cell apoptosis and promote the repair of injury after IRI in mice. Moreover, β-arrestin-2 inhibits autophagy by up-regulating the PI3K/Akt signal to alleviate liver IRI in mice.
