Preliminary study of cytomegalovirus infection and its correlation with NK cell subsets after renal transplantation
10.3969/j.issn.1674-7445.2020.06.006
- VernacularTitle:肾移植术后巨细胞病毒感染及其与NK细胞亚群相关性的初步探讨
- Author:
Shu SUN
1
;
Zhijia LIU
;
Xiang LI
;
Hailong JIN
;
Congran LI
;
Changqing CHEN
;
Bingyi SHI
Author Information
1. Organ Transplantation Institute of 8th Medical Center of Chinese PLA General Hospital, Beijing 100091, China
- Publication Type:Research Article
- Keywords:
Natural killer cell;
Renal transplantation;
Rejection;
Cytomegalovirus;
Infection;
Immunosuppressant;
CD226;
CD16
- From:
Organ Transplantation
2020;11(6):685-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the variation trend of natural killer (NK) cell subsets in the recipients infected with cytomegalovirus (CMV) after renal transplantation. Methods Clinical data of 92 renal transplant recipients were retrospectively analyzed. All recipients were divided into the CMV infection group (n=43), CMV infection recovery group (n=13), stable renal function group (n=15), rejection group (n=11) and other infection group (n=10). In addition, healthy adult volunteers were enrolled in the healthy control group (n=15). The proportion of NK cells in peripheral blood, the expression proportion and the mean fluorescence intensity (MFI) of CD226 and CD16 in NK cells were observed and statistically compared among different groups. Results The proportion of NK cells was 4.9% (2.2%, 11.5%) in the CMV infection group and 3.7% (2.3%, 6.5%) in the CMV infection recovery group, which were significantly lower than those in the other groups (all P < 0.05). The expression proportion of CD226 and CD16 in NK cells in the CMV infection group was significantly lower compared with those in the healthy control group and stable renal function group(all P < 0.05). The expression proportion of CD226 and CD16 in NK cells in the CMV infection recovery group was remarkably higher than those in the CMV infection group (both P < 0.05). The MFI of CD226 and CD16 in the CMV infection group was significantly lower than those in the healthy control group (both P < 0.05). The MFI of CD226 and CD16 in the CMV infection recovery group was significantly higher than those in the CMV infection group (both P < 0.05). Conclusions The expression proportion and MFI of CD226 and CD16 in NK cells are down-regulated in CMV infection period, whereas up-regulated during the CMV infection recovery period, prompting that CD226 and CD16 expressed by NK cells are intimately correlated with the course of CMV infection.
