Simultaneous Determination of 4 Components in Huanshao Capsules by HPLC
- VernacularTitle:HPLC法同时测定还少胶囊中4种成分的含量
- Author:
Xiangde ZHOU
1
;
Xiaolan HUANG
1
;
Wenwu YANG
1
;
Hailing LI
2
;
Nong ZHOU
2
;
Longqiong ZOU
3
;
Li WANG
3
Author Information
1. Chongqing Wanzhou Institute for Food and Drug Control，Chongqing 404047，China
2. College of Biology and Food Engineering，Chongqing Three Gorges University，Chongqing 404120，China
3. Chongqing Three Gorges Yunhai Pharmaceutical Co.，Ltd.，Chongqing 404500，China
- Publication Type:Journal Article
- Keywords:
Huanshao capsules;
HPLC;
Morroniside;
Loganin;
Echinacoside;
Acteoside;
Content determination
- From:
China Pharmacy
2020;31(20):2508-2511
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To estab lish a me thod for simultaneous determination of morroniside ，loganin，echinacoside and acteoside in Huanshao capsules. METHODS ：HPLC method was adopted. The determination was performed on Zhongpuhong RD-C18 column with mobile phase consisted of acetonitrile- 0.1％ formic acid solution （gradient elution ）at the flow rate of 1.0 mL/min. The detection wavelength was set at 240 nm （morroniside，loganin） and 330 nm （echinacoside，acteoside）. The column temperature was set at 35 ℃，and sample size was 10 μL. RESULTS：The linear range were 5.29-105.80 μg/mL for morroniside， 4.49-89.88 mg/L for loganin ，16.26-325.25 mg/L for echinacoside and 16.31-326.25 mg/L for acteoside ，r values were 0.999 9. RSDs of precision ，stability （24 h），reproducibility and durability tests were all lower than 2.0％ . The recoveries were 94.34％ -96.23％（RSD＝0.81％ ，n＝6），97.04％ -98.89％（RSD＝0.73％ ，n＝6），96.23％ -98.08％（RSD＝0.82％ ，n＝6）， 95.40％-98.47％（RSD＝1.23％，n＝6），respectively. The contents of above 4 components in 11 batches of Huanshao Capsules were 0.190-0.704，0.439-0.857，2.723-4.475 and 0.589-1.035 mg/g，respectively. CONCLUSIONS ：Established method is specific ， precise and can be used for content determination of 4 components in Huanshao capsules.
