Analysis of Chemical Compositions in Miao Medicine Caesalpinia decapetala by UPLC-Q-TOF-MS/MS
- VernacularTitle:UPLC-Q-TOF-MS/MS分析苗药云实皮的化学成分
- Author:
Yuan LUO
1
;
Changquan WANG
2
,
3
;
Zipeng GONG
2
;
Yueting LI
2
;
Aimin WANG
4
;
Siying CHEN
2
;
Yong HUANG
2
;
Lin ZHENG
2
Author Information
1. Dept. of Pharmacy，Guizhou Orthopedics Hospital，Guiyang 550002，China
2. Guizhou Provincial Key Laboratory of Pharmaceutics/State Key Laboratory of Functions and Applications of Medicinal Plants，Guizhou Medical University，Guiyang 550004，China
3. School of Pharmacy，Guizhou Medical University，Guiyang 550004，China
4. Engineering Research Center for the Development and Application of Ethnic Medicine and TCM （Ministry of Education ），Guizhou Medical University，Guiyang 550004，China
- Publication Type:Journal Article
- Keywords:
Miao medicine;
Caesalpinia decapetala;
UPLC-Q-TOF-MS/MS;
Chemical composition;
Analysis and
- From:
China Pharmacy
2020;31(20):2481-2486
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To estab lish a method that can comprehensively and rapidly analyze the chemical compositions of Miao medicine Caesalpinia decapetala，and to providing reference for quality control and pharmacodynamic material basis study of C. decapetala . METHODS ：UPLC-Q-TOF-MS/MS was adopted . The determination was performed on Agilent SB-C 18 column with mobile phase consisted of 0.1％ formic acid solution- 0.1％ formic acid acetonitrile （gradient elution ）at the flow rate of 0.25 mL/min. The column temperature was 30 ℃，and sample size was 2 µL. ESI source was applied in negative and positive scanning ion mode and data collection range of m/z 50-1 500. The capillary voltage was 4.5 kV，the atomizing gas （nitrogen）pressure was 1.2 Bar， the solvent removal gas was nitrogen ，the flow rate of solvent removal gas was 8 L/min and the solvent removal gas temperature was 200 ℃. Data Analysis 4.2 software was adopted to analyze fragment ion information of each peak ，and identify chemica l compositions on the basis of relevant literature and mass spectograms of reference substance. RESULTS ：Under positive ion mode ， 9 chemical compounds were identified ；peak 1，2，3，4，5，6，7，8，9 were catechin ，protohematoxylin B ，epicatechin，ethyl gallate，quercetin，luteolin，3-deoxy-hematoxylin chalcone ， isoliquiritigenin and linoleic acid. Under negative ion mode ， U1812403）， totally 21 peaks were confirmed and 13 compounds were identified；peak 3，4，5，6，7，8，9，10，11，12，13，15， 21 were catechins ， brevifolin carboxylic acid ， proto- hematoxylin B ，epicatechin，ethyl gallate ，epicatechin gallate ， quercetin，resveratrol，hematoxylin，luteolin，3-deoxy-hema- toxylin， isoliquiritigenin， linoleic acid. CONCLUSIONS UPLC-Q-TOF-MS/MS method is established successfully for analysis of chemical compositions in C. decapetala .
