Study on the Extraction Technology of Fructus Gleditisae Abnormalis and GC-MS Analysis of Extracted Com- ponents
- VernacularTitle:猪牙皂的萃取方法研究及萃取成分的GC-MS分析
- Author:
Bili DENG
1
;
Nana DONG
1
;
Xulan WANG
1
;
Chengyue LUO
1
;
Xiaolan CHEN
1
Author Information
1. College of Pharmacy，Guizhou University of TCM，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Fructus Gleditisae Abnormalis;
CO2 supercritical fluid extraction;
Single factor test;
Orthogonal test;
GC-MS
- From:
China Pharmacy
2020;31(20):2469-2472
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish and optimize a extraction method of Fructus Gleditsiae Abnormallis ，and to analyze and identify chemical components of the extract simultaneously. METHODS ：Fructus Gleditsiae Abnormallis was extracted with CO 2 supercritical fluid extraction （SFE）method. Based on single factor tests ，using extraction yield as index ，extraction temperature ， extraction pressure and extraction time as investigation factors ，SFE technology was optimized with orthogonal test ，and validation test was performed. Chemical components in the extract were identified by GC-MS. Relative percentage of each component was calculated with area normalization method. RESULTS ：The optimal SFE extraction technology of Fructus Gleditsiae Abnormallis was extraction temperature of 60 ℃，extraction pressure of 300 MPa and pression time of 15 min. Average extraction of 3 times of validation tests was 1.73％（RSD＝1.78％，n＝3）. The 48 components in the extracts of Fructus Gleditsiae Abnormallis were identified，which accounted for 98.31％ of the total amount of the extracts. The extracts of Fructus Gleditisae Abnormalis mainly included organic acids ，accounting for 36.99％，followed by alkaloids ，accounting for 12.59％ in total. Main components were palmitic acid （16.62％），oleic acid （14.12％），N-aminotetrahydropyrrole（9.79％），2，6-dimethyloctane-1，7-dien-3-ol（5.95％）， tetrahydropyran（3.83％），vanillin（3.39％），etc. CONCLUSIONS ：SFE method of Fructus Gleditisae Abnormalis is established successfully，and the extract is mainly organic acids.
