Expression level of lncRNA SNHG1 in endometrial cancer tissues and its clinical significance
10.3872/j.issn.1007-385x.2020.10.008
- VernacularTitle:lncRNA SNHG1在子宫内膜癌组织中的表达水平及其临床意义
- Author:
ZHU Xiaoling
1
;
LIU Shumei
1
;
YANG Aihua
2
,
3
,
4
;
TAO Anfu
5
,
6
Author Information
1. 1. Department of Gynaecology and Obstetrics, Zhongxin Ecocity Hospital of Tianjin Medical University, Tianjin 300467, China
2.
3. 2. Department of Gynaecology and Obstetrics, Tianjin Haibin People&rsquo
4. s Hospital, Tianjin 300450, China
5. 3. Department of Clinical Laboratory, Tianjin Medical University Cancer Hospital, National Clinical ResearchCenter for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin'
6. s Clinical Research Center for Malignant Cancer, Tianjin300060, China
- Publication Type:Journal Article
- Keywords:
lncRNA SNHG1;
RELA;
NF-κB;
endometrial cancer;
HEC-1A cell;
proliferation;
migration;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2020;27(10):1118-1125
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the expression level of lncRNA (long non-coding RNA) SNHG1 in endometrial cancer tissues,
and to analyze its mechanism of action as well as its clinical significance. Methods: NCBI-GEO and TCGA database were used to
analyze the expression level of SNHG1 in endometrial cancer. A total of 53 cases of endometrial cancer tissue samples and 41 cases
of normal endometrial tissue samples were collected from January 2016 to March 2019 at Zhongxin Ecocity Hospital of Tianjin Medical
University; in addition, endometrial cancer cell lines Ishikawa and HEC-1A as well as normal endometrial ESC cells were also collected for
this study. qPCR was used to detect the expression level of SNHG1 in tissues and cells, and its correlation with the clinical characteristics
of patients were statistically analyzed. The effect of SNHG1 on cell proliferation and apoptosis of HEC-1A cells was measured by
MTT assay and Annexin V/PI double staining Flow cytometry, respectively. The migration and invasion of HEC-1A cells were
measured by Transwell assay. StarBase was used to predict the regulatory relationship between SNHG1 and RELA, which was then
verified by qPCR and Western blotting. Dual fluorescent reporter gene system and qPCR were adopted to identify the influence of
SNHG1 on NF-κB pathway. Results: The expression of SNHG1 was significantly up-regulated in endometrial cancer tissues compared
with normal endometrial tissues (P<0.01), and its expression was related to tumor size, TNM staging, histological grade and lymph node metastasis (all P<0.05). The expression level of SNHG1 in Ishikawa and HEC-1A cells was significantly higher than that in ESC
cells (all P<0.01). Overexpression of SNHG1 notably promoted the proliferation, migration and invasion and inhibited cell apoptosis of
HEC-1A cells. By promoting the expression of RELA, SNHG1 activated the NF-κB pathway and promoted the expressions of downstream gene IL-6 and CCL19 (all P<0.01). Conclusion: Up-regulated SNHG1 in endometrial cancer functions as an oncogene by
activating the NF-κB pathway through promoting the RELA expression.
- Full text:20201008.pdf
