Effect of lncRNA SNHG15 targeting miR-153 on apoptotic of mitochondrial pathway in breast cancer cells
10.3872/j.issn.1007-385x.2020.10.003
- VernacularTitle:lncRNA SNHG15靶向miR-153对乳腺癌细胞线粒体途径凋亡的影响
- Author:
WU Chao
1
;
WANG Caixing
1
;
HAN Yujiao
1
;
NIU Guifang
1
Author Information
1. (Department of Clinical Surgery, Shanxi University of Traditional Chinese Medicine,
- Publication Type:Journal Article
- Keywords:
lncRNA SNHG15;
miR-153;
breast cancer;
MDA-MB-231 cell;
apoptosis;
mitochondrial pathway
- From:
Chinese Journal of Cancer Biotherapy
2020;27(10):1087-1092
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effect of lncRNA SNHG15 targeting miR-153 on cell viability and apoptosis of breast cancer cells and its apoptotic mechanism. Methods:The expression of SNHG15 in breast cancer cell lines(MDA-MB-231, BT-549 and MCF-7)
were detected by Real-time fluorescent quantitative PCR (qPCR). MDA-MB-231 cells were divided into control (Ctrl) group, si-NC group, si-SNHG15 group, si-SNHG15+anti-NC group and si-SNHG15+anti-miR-153 group. Cell viability and apoptosis rate were detected by MTT and Flow cytometry, respectively. The targeting relationship between SNHG15 and miR-153 was verified by Dual luciferase report gene system. Mitochondrial membrane potential fluorescent probe (JC-1) staining method was used to detect cell mitochondrial membrane potential. The expressions of mitochondrial apoptosis-related proteins (Bcl-2, Bax, caspase3, cleaved caspase3
[c-caspase3] and Cyt-C）were detected by Western blotting. Results: The expression of SNHG15 in breast cancer cells was significantly
higher than that in human normal mammary epithelial MCF10A cells (P<0.01). There was a targeting relationship between SNHG15
and miR-153. Compared with the control group, the cell viability and mitochondrial membrane potential of MDA-MB-231 cells in si-SNHG15 group were decreased, while apoptosis rate was increased (all P<0.01); the expressions of Bcl-2 and caspase3 were
decreased while expressions of Bax, c-caspase3 and Cyt-C were increased (all P<0.01). However, co-transfection of si-SNHG15 and
anti-miR-153 significantly attenuated the effects of si-SNHG15 on cell viability, apoptosis, mitochondrial membrane potential and
expressions of Bcl-2, Bax, caspase3, c-caspase3 and Cyt-C (all P<0.01). Conclusion: lncRNA SNHG15 can target miR-153 to induce
apoptosis of MDA-MB-231 cells, and the mechanism may be related to the regulation of apoptosis of mitochondrial pathway.
- Full text:20201003.pdf
