Monoclonal antibody 18H12 suppresses the self-renewal and invasion of PAMC-82 gastric cancer stem cells
10.3872/j.issn.1007-385x.2020.10.002
- VernacularTitle:单克隆抗体18H12抑制PAMC-82胃癌干细胞的自我更新和侵袭能力
- Author:
YANG Ting
1
;
SHU Xiong
2
,
3
;
SUN Lixin
1
;
YU Long
1
;
SUN Lichao
1
;
YANG Zhihua
1
;
RAN Yuliang
1
Author Information
1. National Cancer Center, National Cancer Clinical Medical Research Center, State Key Laboratory of Molecular Oncology, Cancer Hospital of Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
2. Beijing Jishuitan Hospital &
3. Beijing Orthopaedic Trauma Research Institute, Beijing 100021, China
- Publication Type:Journal Article
- Keywords:
gastric cancer stem cell;
monoclonal antibody 18H12;
PAMC-82 cell;
self-renewal;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2020;27(10):1081-1086
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To investigate the effect of 18H12, a functional monoclonal antibody that can target gastric cancer stem cells, on the self-renewal and invasion ability of gastric cancer cells. Methods: The gastric cancer cell line PAMC-82 was used as cell model, the expression of ENO1 (enolase-1) on the membrane surface of its parental cells and enriched stem cells by sphere culture was detected by Flow cytometry. Flow cytometry was used to separate ENO1+ cells and ENO1-
cells to detect their self-renewal ability and invasion ability. With the commercial ENO1 antigen and antibody as the samples, CoIP (co-immunoprecipitation) was used to verify whether 18H12
antibody targeting ENO1 could able to accurately recognize ENO1. After being treated with 18H12 for 12 h, 24 h and 48 h, the selfrenewal and invasion ability of PAMC-82 cells were detected by methylcellulose pelletization experiment and Transwell chamber assay, respectively. Results: Flow cytometry showed that the expression of ENO1 on the membrane surface of PAMC-82 sphere cells was significantly higher than that of its parental cells (P<0.01), so ENO1 could be a potential target for targeting gastric cancer stem cells. The self-renewal ability and invasion ability of the sorted ENO1+ cells were significantly stronger than those of the ENO1-
cells and the parental cells (P<0.05 or P<0.01). 18H12 antibody could accurately recognize ENO1, which was consistent with the commercial antibody recognition band. 18H12 could significantly inhibit self-renewal ability and invasion ability of PAMC-82 cells (P<0.01). Conclusion: Monoclonal antibody 18H12 can significantly inhibit the self-renewal and invasion of gastric cancer stem cells and is expected to be a candidate antibody drug targeting gastric cancer stem cells.
- Full text:20201002.pdf
