Effect of cyclooxygenase-2 inhibitors on the expression of the Acsl gene family in the ileum of rats with nonalcoholic fatty liver disease
DOI:10.3969/j.issn.1001-5256.2020.09.026
- VernacularTitle:环氧合酶2抑制剂对非酒精性脂肪性肝病大鼠模型回肠Acsl基因家族表达的影响
- Author:
Shan GUO
1
;
Shijie YI
;
Xuefeng YANG
;
Ting CAO
;
Nian FU
;
Kebing ZHOU
;
Jianwu LONG
Author Information
1. Department of Gastroenterology, Nanhua Hospital Affiliated to Nanhua University, Hengyang, Hunan 421002, China
- Publication Type:Research Article
- Keywords:
cyclooxygenase 2;
non-alcoholic fatty liver disease;
rats, sprague-dawley;
genes
- From:
Journal of Clinical Hepatology
2020;36(9):2040-2044
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect and significance of cyclooxygenase-2 (COX-2) inhibitors on the expression of the Acsl gene family in the ileum of rats with nonalcoholic fatty liver disease (NAFLD). MethodsA total of 45 Sprague-Dawley rats were randomly divided into normal control group (15 rats given normal diet), NAFLD model group (15 rats given high-fat diet), and nimesulide group (15 rats given high-fat diet and nimesulide). All rats were sacrificed after 12 weeks of feeding, and then blood samples were collected from the inferior vena cava to measure total cholesterol (TC) and triglyceride (TG). HE staining and oil red O staining were performed for the liver to evaluate the degree of hepatic steatosis in each group, and quantitative real-time PCR was used to measure the mRNA expression of the Acsl family genes in the ileum. An analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. ResultsCompared with the normal control group, the NAFLD model group had significant increases in serum TC and TG and marked hepatic steatosis (all P＜0.05); compared with the NAFLD model group, the nimesulide group had significant reductions in serum TC and TG and degree of hepatic steatosis (all P＜0.05). Compared with the normal control group, the NAFLD model group had a significant increase in the expression of COX-2 in the ileum (P＜0.05), and compared with the NAFLD model group, the nimesulide group had a significant reduction in the expression of COX-2 in the ileum (P＜005). Compared with the normal control group, the NAFLD model group had significant increases in the mRNA expression of Acsl3 and Acsl5 in the ileum (both P＜0.05), and compared with the NAFLD model group, the nimesulide group had significant reductions in the mRNA expression of Acsl3 and Acsl5 (both P＜0.05). ConclusionThe COX-2 inhibitor nimesulide can regulate the expression of the Acsl gene family in the ileum of rats with NAFLD, suggesting that COX-2 inhibitors may inhibit the progression of NAFLD through the Acsl gene.
