Effect of electroacupuncture of " acupoint combination" on central sensitivity of cholecystokinin in obese rats with insulin resistance.
10.13703/j.0255-2930.20190515-k0003
- Author:
Ai-Qun SONG
1
,
2
;
Yang-Pu ZHANG
3
;
Min YAO
1
,
2
;
Feng-Xia LIANG
4
Author Information
1. Department of Acupuncture and Moxibustion, Hubei Hospital of TCM, Wuhan 430061, China
2. Hubei Academy of TCM, Wuhan 430074.
3. Rehabilitation Medicine Center of Hubei Hospital of Integrated Chinese and Western Medicine.
4. College of Acupuncture-Moxibustion and Orthopedics, Hubei University of CM, Hubei Collaborative Innovation Center of Acupuncture and Moxibustion for Disease Prevention, Wuhan 430061.
- Publication Type:Journal Article
- Keywords:
central sensitivity;
cholecystokinin (CCK);
electroacupuncture;
insulin resistance (IR);
obesity
- From:
Chinese Acupuncture & Moxibustion
2020;40(9):969-975
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of electroacupuncture (EA) of " acupoint combination" on appetite, body fat, insulin sensitivity and central sensitivity of cholecystokinin (CCK) in obese rats with insulin resistance (IR), and to explore the mechanism of EA on improving obesity with insulin resistance.
METHODS:Among the fifty 8-week-old healthy SPF male Wistar rats, 10 rats were randomly selected and fed with normal diet; after 8 weeks, 8 rats were randomly selected as a normal group. The remaining 40 rats were fed with high-fat diet to establish the model of obsesity IR; after 8 weeks, 24 rats with successful model of obsesity IR were randomly divided into a model group, an EA group and a sham EA group, 8 rats in each group. Eight weeks after model establishment, the rats in the EA group were intervened with EA at "Fenglong" (ST 40), "Zhongwan" (CV 12), "Guanyuan" (CV 4) and "Zusanli" (ST 36), with continuous wave, in frequency of 2 Hz, and current intensity of 1 mA, for 10 min each time. The rats in the sham EA group were intervened with EA at the points 5 mm next to the acupoints used in the EA group and no electricity was given; the sham EA was given for 10 min each time. Both the treatments were given once every other day for 8 weeks. The Lee's index and food intake were observed before the intervention as well as 2 weeks, 4 weeks, 6 weeks and 8 weeks into intervention; after the intervention, serum insulin (INS) and glucose infusion rate (GIR) were detected; serum cholecystokinin (CCK) level was detected by ELISA; c-fos expression in the area postrema (AP) and nucleus tractus solitarius (NTS) of medulla oblongata was detected by immunohistochemistry.
RESULTS:Before the intervention as well as 2 weeks, 4 weeks, 6 weeks and 8 weeks into intervention, the Lee's index and food intake in the model group were higher than those in normal group (<0.01). The Lee's index (6 weeks and 8 weeks into intervention) and food intake (4 weeks, 6 weeks and 8 weeks into intervention) in the EA group were lower than those in the model group and the sham EA group (<0.05, <0.01). After intervention, compared with the normal group, serum level of INS was increased (<0.01), while GIR, serum CCK level, c-fos expression in AP and NTS in the model group were decreased (<0.01, <0.05). Compared with the model group and the sham EA group, serum level of INS in the EA group was decreased (<0.01), and GIR, serum CCK level, c-fos expression in AP and NTS were increased (<0.01, <0.05).
CONCLUSION:EA of " acupoint combination" could effectively reduce appetite, body fat and enhance insulin sensitivity in obese rats with IR. The mechanism may be related to the regulation of central sensitivity of CCK.
