Dihydroartemisinin Induces Apoptosis of Human Acute T Lymphocytic Leukemia Cells by Activating Oxidative Stress.
10.19746/j.cnki.issn.1009-2137.2020.03.007
- Author:
Wei-Dong SUN
1
;
Xing-Xing YU
1
;
Yi-Han AN
1
;
Xin WANG
2
;
Ying WANG
2
;
Xiang-Min TONG
3
Author Information
1. Graduate School of Bengbu Medical College, Bengbu 233000, Anhui Province, China,Key Laboratory of Molecular Diagnosis and Individualization of Cancer, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang Province,China.
2. Key Laboratory of Molecular Diagnosis and Individualization of Cancer, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang Province,China.
3. Key Laboratory of Molecular Diagnosis and Individualization of Cancer, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang Province,China,E-mail: tongxiangmin11@163.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Artemisinins;
Humans;
Jurkat Cells;
Oxidative Stress;
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma;
Reactive Oxygen Species
- From:
Journal of Experimental Hematology
2020;28(3):753-757
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of dihydroartemisinin (DHA) on the proliferation and apoptosis of human T-cell acute lymphoblastic leukemia (T-ALL) Jurkat cell.
METHODS:The effects of DHA on the proliferation of Jurkat cells and the recovery of DHA-inhibited cell viability by N-acetyl-L-cysteine (NAC) were examined by CCK-8 assay. Flow cytometry was performed to analyze the cell apoptosis and generation of reactive oxygen species (ROS). Western-blot was used to detected protein expression of DNA damage-related genes, as well as apoptosis-associated genes, respectively.
RESULTS:DHA inhibited the proliferation of Jurkat cells, and shows a concentration-dependent manner(r =0.936), and NAC could partially restore the activity of DHA on cell proliferation inhibition. With the increase of drug concentration, the apoptosis rate (r =0.946) and ROS accumulation was increased (r =0.965). Western blot showed that the protein expressions of DNA damage-related gene γ-H2AX and apoptosis-related genes p53, c-Caspase3, BAX and cPARP were significantly increased, and BCL-2 protein expression was decreased.
CONCLUSION:DHA can induce ROS production in Jurkat cells, which can cause DNA damage, activate the P53 apoptotic pathway, and promote apoptosis of cells.
