Interferon-induced Transmembrane Protein 3 Prevents Acute Influenza Pathogenesis in Mice.

Qiang Sun; Na Lei; Jian LU; Rong Bao Gao; Zi LI; Li Qi Liu; Ying Sun; Jun Feng Guo; Da Yan Wang; Yue Long Shu

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Interferon-induced Transmembrane Protein 3 Prevents Acute Influenza Pathogenesis in Mice.

Author: Qiang SUN ^{1
,

2} ; Na LEI ^{3
,

4} ; Jian LU ⁵ ; Rong Bao GAO ⁵ ; Zi LI ⁵ ; Li Qi LIU ⁵ ; Ying SUN ⁵ ; Jun Feng GUO ⁵ ; Da Yan WANG ⁵ ; Yue Long SHU ^{1
,

2}
Author Information

1. School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen 510275, Guangdong, China
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing 102206, China.
3. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing 102206, China
4. Chaoyang District Center for Disease Prevention and Control, Beijing 100021, China.
5. School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen 510275, Guangdong, China.
Publication Type:Journal Article
Keywords: IFITM3; Immune response; Influenza; NK cells
MeSH: Acute Disease; Animals; Disease Models, Animal; Female; Humans; Influenza, Human; virology; Male; Membrane Proteins; genetics; metabolism; Mice; Mice, Inbred C57BL; Orthomyxoviridae Infections; veterinary; virology; Rodent Diseases; virology
From: Biomedical and Environmental Sciences 2020;33(5):295-305
CountryChina
Language:English
Abstract: Objective:Interferon-induced transmembrane protein 3 (IFITM3) is an important member of the IFITM family. However, the molecular mechanisms underlying its antiviral action have not been completely elucidated. Recent studies on IFITM3, particularly those focused on innate antiviral defense mechanisms, have shown that IFITM3 affects the body's adaptive immune response. The aim of this study was to determine the contribution of IFITM3 proteins to immune control of influenza infection .
Methods:We performed proteomics, flow cytometry, and immunohistochemistry analysis and used bioinformatics tools to systematically compare and analyze the differences in natural killer (NK) cell numbers, their activation, and their immune function in the lungs of -/- and wild-type mice.
Results:-/- mice developed more severe inflammation and apoptotic responses compared to wild-type mice. Moreover, the NK cell activation was higher in the lungs of -/- mice during acute influenza infection.
Conclusions:Based on our results, we speculate that the NK cells are more readily activated in the absence of IFITM3, increasing mortality in -/- mice.