Distribution of Microbiota in Fine Particulate Matter Particles in Guangzhou, China.

Shi Rui Dong; Ya Jing Han; Jing WU; Cheng Li Zeng; Ke Hui Zhu; Xiao Jing Chen; Yu Mei Liu; Xiao Qian Zou; Shao Ling Zheng; Zi Hao Wen; Dan Dan Liu; Yao Wang; Xiu Xia Huang; Xiu Ben DU; Jian Lei Hao; Huan Yu Wang; Shu Guo; Chun Xia Jing; Guang Yang

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Distribution of Microbiota in Fine Particulate Matter Particles in Guangzhou, China.

Author: Shi Rui DONG ^{1
,

2} ; Ya Jing HAN ^{1
,

3
,

4} ; Jing WU ^{1
,

3
,

4} ; Cheng Li ZENG ⁵ ; Ke Hui ZHU ^{1
,

2} ; Xiao Jing CHEN ^{1
,

2} ; Yu Mei LIU ^{1
,

2} ; Xiao Qian ZOU ^{1
,

2} ; Shao Ling ZHENG ⁵ ; Zi Hao WEN ⁵ ; Dan Dan LIU ⁵ ; Yao WANG ⁵ ; Xiu Xia HUANG ⁵ ; Xiu Ben DU ⁵ ; Jian Lei HAO ^{6
,

7} ; Huan Yu WANG ⁸ ; Shu GUO ⁴ ; Chun Xia JING ^{3
,

9
,

10} ; Guang YANG ^{1
,

3
,

10}
Author Information

1. Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, Guangdong, China
2. Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, Guangdong, China.
3. Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, Guangdong, China
4. Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, Guangdong, China.
5. Department of Epidemiology, School of Medicine, Jinan University, Guangzhou 510632, Guangdong, China.
6. Zhuhai Precision Medical Center, Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Jinan University, Zhuhai 519000, Guangdong, China
7. The Biomedical Translational Research Institute, Faculty of Medical Science, Jinan University, Guangzhou 510632, Guangdong, China.
8. Department of Viral Encephalitis, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
9. Department of Epidemiology, School of Medicine, Jinan University, Guangzhou 510632, Guangdong, China
10. The Key Laboratory for Virology of Guangzhou, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China.
Publication Type:Journal Article
Keywords: Diversity; Guangzhou; Microbiota; PM2.5
MeSH: Air Microbiology; Air Pollutants; analysis; Bacteria; classification; isolation & purification; China; Cities; Environmental Monitoring; Microbiota; Particle Size; Particulate Matter; analysis; RNA, Bacterial; analysis; RNA, Ribosomal, 16S; analysis
From: Biomedical and Environmental Sciences 2020;33(5):306-314
CountryChina
Language:English
Abstract: Objective:High PM concentration is the main feature of increasing haze in developing states, but information on its microbial composition remains very limited. This study aimed to determine the composition of microbiota in PM in Guangzhou, a city located in the tropics in China.
Methods:In Guangzhou, from March 5 to 10 , 2016, PM was collected in middle volume air samplers for 23 h daily. The 16S rDNA V4 region of the PM sample extracted DNA was investigated using high-throughput sequence.
Results:Among the Guangzhou samples, , , , , and were the dominant microbiota accounting for more than 90% of the total microbiota, and was the dominant gram-negative bacteria, accounting for 21.30%-23.57%. We examined the difference in bacterial distribution of PM between Beijing and Guangzhou at the genus level; was found in both studies, but was only detected in Guangzhou.
Conclusion:In conclusion, the diversity and specificity of microbial components in Guangzhou PM were studied, which may provide a basis for future pathogenicity research in the tropics.