Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Isolates from Clinical Patients, Tap Water Systems, and Food.

Shuang Meng; Yong Lu Wang; Chengeng Liu; Jing Yang; Min Yuan; Xiang Ning Bai; Dong Jin; Jun Rong Liang; Zhi Gang Cui; Juan LI

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Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Isolates from Clinical Patients, Tap Water Systems, and Food.

Author: Shuang MENG ^{1
,

2} ; Yong Lu WANG ³ ; ChenGeng LIU ⁴ ; Jing YANG ^{1
,

2} ; Min YUAN ^{1
,

2} ; Xiang Ning BAI ^{1
,

2} ; Dong JIN ^{1
,

2} ; Jun Rong LIANG ^{1
,

2} ; Zhi Gang CUI ^{1
,

2} ; Juan LI ^{1
,

2}
Author Information

1. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 10026, China
2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang 310058, Hangzhou, China.
3. Ma'anshan Center for Disease Control and Prevention, Ma'anshan 243031, Anhui, China.
4. Clinical Laboratory of Xuanwu Hospital, Captital Medcial University, Beijing 100053, China.
Publication Type:Journal Article
Keywords: Aeromonas; Antimicrobial resistance gene; Multi-locus sequence typing; Multidrug resistance; Virulence gene
MeSH: Aeromonas; drug effects; genetics; isolation & purification; pathogenicity; Anti-Bacterial Agents; pharmacology; China; Drinking Water; microbiology; Drug Resistance, Bacterial; Food Microbiology; Genetic Variation; Gram-Negative Bacterial Infections; microbiology; Species Specificity; Virulence
From: Biomedical and Environmental Sciences 2020;33(6):385-395
CountryChina
Language:English
Abstract: Objective:This study aimed to evaluate the genetic diversity, virulence, and antimicrobial resistance of isolates from clinical patients, tap water systems, and food.
Methods:Ninety isolates were obtained from Ma'anshan, Anhui province, China, and subjected to multi-locus sequence typing (MLST) with six housekeeping genes. Their taxonomy was investigated using concatenated sequences, while their resistance to 12 antibiotics was evaluated. Ten putative virulence factors and several resistance genes were identified by PCR and sequencing.
Results:The 90 isolates were divided into 84 sequence types, 80 of which were novel, indicating high genetic diversity. The isolates were classified into eight different species. PCR assays identified virulence genes in the isolates, with the enterotoxin and hemolysin genes , , , and found in 47 (52.2%), 13 (14.4%), 22 (24.4%), and 12 (13.3%) of the isolates, respectively. The majority of the isolates (≥ 90%) were susceptible to aztreonam, imipenem, cefepime, chloramphenicol, gentamicin, tetracycline, and ciprofloxacin. However, several resistance genes were detected in the isolates, as well as a new variant.
Conclusions:Sequence type, virulence properties, and antibiotic resistance vary in isolates from clinical patients, tap water systems, and food.