Photothermal Effect-based Cytotoxic Ability of Melanin from Shells to Heal Wounds Infected with Drug-resistant Bacteria .
- Author:
Ya Mei LIU
1
;
Wei Shuai MA
1
;
Yu Xi WEI
1
;
Yuan Hong XU
2
,
3
Author Information
- Publication Type:Journal Article
- Keywords: Antibacterial; Melanin; Photothermal conversion; Wound healing
- MeSH: Animal Shells; chemistry; Animals; Anti-Bacterial Agents; pharmacology; Escherichia coli; drug effects; radiation effects; Escherichia coli Infections; drug therapy; Melanins; pharmacology; Mytilus edulis; chemistry; Photochemical Processes; Rats; Rats, Sprague-Dawley; Staphylococcal Infections; drug therapy; Staphylococcus aureus; drug effects; radiation effects; Wound Healing
- From: Biomedical and Environmental Sciences 2020;33(7):471-483
- CountryChina
- Language:English
-
Abstract:
Objective:Owing to antibiotic abuse and the subsequent development of antibiotic resistance, bacterial infection has become one of the most persistent unresolved problems. New antibacterial agents, especially those that are environmental-friendly, are urgently needed.
Methods:Melanin extracted by filtration centrifugation and acid and proteolytic hydrolysis was characterized using UV, FTIR, TEM, and XPS. Photothermal conversion was calculated, and the bacteriostatic effects, and , were assessed by plate counting and ratios (%) of wound areas.
Results:Natural melanin hydrolyzed by trypsin had good photothermal conversion effects, which resulted in superior bacteriostatic activities. The extracted melanin along with laser NIR irradiation at 808 nm promoted the healing of wounds infected by drug-resistant bacteria and was biocompatible according to toxicity tests and .
Conclusion:The present findings indicated a safe and efficient method of developing natural antibacterial agents.
