Assessment of the Sensitivity and Specificity of the Established Real-time Quaking-induced Conversion (RT-QuIC) Technique in Chinese CJD Surveillance.

Kang Xiao; Xue Hua Yang; Wen Quan Zou; Xiao Ping Dong; Qi Shi

Return

Assessment of the Sensitivity and Specificity of the Established Real-time Quaking-induced Conversion (RT-QuIC) Technique in Chinese CJD Surveillance.

Author: Kang XIAO ¹ ; Xue Hua YANG ¹ ; Wen Quan ZOU ¹ ; Xiao Ping DONG ^{2
,

3
,

4} ; Qi SHI ¹
Author Information

1. State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University), National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University), National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
3. Center for Global Public Health, Chinese Center for Disease Control and Prevention, Beijing 102206, China
4. Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, Hubei China.
Publication Type:Letter
MeSH: China; Creutzfeldt-Jakob Syndrome; diagnosis; Diagnostic Tests, Routine; methods; Humans; PrPSc Proteins; cerebrospinal fluid; Sensitivity and Specificity
From: Biomedical and Environmental Sciences 2020;33(8):620-622
CountryChina
Language:English
Abstract: Real-time quaking-induced conversion (RT-QuIC) assay is a newly established PrP -detecting method. The development of RT-QuIC improves the diagnosis of sporadic Creutzfeldt-Jakob disease (sCJD), showing good sensitivity and specificity in many countries when the method was used in cerebrospinal fluid (CSF) samples. However, in China, the sensitivity and specificity of RT-QuIC has yet to be determined due to the lack of definitive diagnosis samples. Recently, 30 definitive sCJD and 30 non-CJD diagnoses were evaluated by RT-QuIC assay. In the 30 sCJD CSF samples, 29 showed positive results. By contrast, all the non-CJD samples were negative. The sensitivity and specificity of our RT-QuIC assay were 96.67% and 100%, respectively, and are comparable to other published data. Results can provide a fundamental basis for the usage of RT-QuIC assay in CJD surveillance in China.