Characteristics of oral methicillin-resistant Staphylococcus epidermidis isolated from dental plaque.
10.1038/s41368-020-0079-5
- Author:
Boyu TANG
1
;
Tao GONG
1
;
Yujia CUI
1
;
Lingyun WANG
2
;
Chao HE
3
;
Miao LU
1
;
Jiamin CHEN
1
;
Meiling JING
1
;
Anqi ZHANG
1
;
Yuqing LI
4
Author Information
1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu, China.
2. Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
3. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu, China. liyuqing@scu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Anti-Bacterial Agents;
therapeutic use;
Dental Plaque;
microbiology;
Female;
Humans;
Methicillin;
Methicillin-Resistant Staphylococcus aureus;
isolation & purification;
Staphylococcal Infections;
diagnosis;
Staphylococcus epidermidis;
isolation & purification
- From:
International Journal of Oral Science
2020;12(1):15-15
- CountryChina
- Language:English
-
Abstract:
The oral microbial community is widely regarded as a latent reservoir of antibiotic resistance genes. This study assessed the molecular epidemiology, susceptibility profile, and resistance mechanisms of 35 methicillin-resistant Staphylococcus epidermidis (MRSE) strains isolated from the dental plaque of a healthy human population. Broth microdilution minimum inhibitory concentrations (MICs) revealed that all the isolates were nonsusceptible to oxacillin and penicillin G. Most of them were also resistant to trimethoprim (65.7%) and erythromycin (54.3%). The resistance to multiple antibiotics was found to be largely due to the acquisition of plasmid-borne genes. The mecA and dfrA genes were found in all the isolates, mostly dfrG (80%), aacA-aphD (20%), aadD (28.6%), aphA3 (22.9%), msrA (5.7%), and the ermC gene (14.3%). Classical mutational mechanisms found in these isolates were mainly efflux pumps such as qacA (31.4%), qacC (25.7%), tetK (17.1%), and norA (8.6%). Multilocus sequence type analysis revealed that sequence type 59 (ST59) strains comprised 71.43% of the typed isolates, and the eBURST algorithm clustered STs into the clonal complex 2-II(CC2-II). The staphyloccoccal cassette chromosome mec (SCCmec) type results showed that 25 (71.43%) were assigned to type IV. Moreover, 88.66% of the isolates were found to harbor six or more biofilm-associated genes. The aap, atlE, embp, sdrF, and IS256 genes were detected in all 35 isolates. This research demonstrates that biofilm-positive multiple-antibiotic-resistant ST59-SCCmec IV S. epidermidis strains exist in the dental plaque of healthy people and may be a potential risk for the transmission of antibiotic resistance.
