Berberine mediates root remodeling in an immature tooth with apical periodontitis by regulating stem cells from apical papilla differentiation.
10.1038/s41368-020-0085-7
- Author:
Yujia CUI
1
;
Jing XIE
2
;
Yujie FU
1
;
Chuwen LI
3
;
Liwei ZHENG
4
;
Dingming HUANG
1
;
Changchun ZHOU
5
;
Jianxun SUN
6
;
Xuedong ZHOU
7
Author Information
1. State Key Laboratory of Oral Diseases & National Clinical Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
2. State Key Laboratory of Oral Diseases & National Clinical Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu, China.
3. Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology & National Clinical Research Center for Oral Diseases & Department of Oral and Maxillofacial-Head Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
5. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China.
6. State Key Laboratory of Oral Diseases & National Clinical Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China. jxsun@scu.edu.cn.
7. State Key Laboratory of Oral Diseases & National Clinical Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China. zhouxd@scu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Berberine;
pharmacology;
Cell Differentiation;
drug effects;
Dental Papilla;
Male;
Osteogenesis;
drug effects;
Periapical Periodontitis;
therapy;
Rats;
Stem Cells;
cytology;
drug effects;
metabolism;
Wnt Signaling Pathway;
drug effects;
Wnt3A Protein;
genetics;
metabolism;
X-Ray Microtomography
- From:
International Journal of Oral Science
2020;12(1):18-18
- CountryChina
- Language:English
-
Abstract:
Once pulp necrosis or apical periodontitis occurs on immature teeth, the weak root and open root apex are challenging to clinicians. Berberine (BBR) is a potential medicine for bone disorders, therefore, we proposed to apply BBR in root canals to enhance root repair in immature teeth. An in vivo model of immature teeth with apical periodontitis was established in rats, and root canals were filled with BBR, calcium hydroxide or sterilized saline for 3 weeks. The shape of the roots was analyzed by micro-computed tomography and histological staining. In vitro, BBR was introduced into stem cells from apical papilla (SCAPs). Osteogenic differentiation of stem cells from apical papilla was investigated by alkaline phosphatase activity, mineralization ability, and gene expression of osteogenic makers. The signaling pathway, which regulated the osteogenesis of SCAPs was evaluated by quantitative real time PCR, Western blot analysis, and immunofluorescence. In rats treated with BBR, more tissue was formed, with longer roots, thicker root walls, and smaller apex diameters. In addition, we found that BBR promoted SCAPs osteogenesis in a time-dependent and concentration-dependent manner. BBR induced the expression of β-catenin and enhanced β-catenin entering into the nucleus, to up-regulate more runt-related nuclear factor 2 downstream. BBR enhanced root repair in immature teeth with apical periodontitis by activating the canonical Wnt/β-catenin pathway in SCAPs.
