Molecular mechanism underlying the inhibitory effect of propofol on lipopolysaccharide-induced pyroptosis of mouse bone marrow-derived macrophages.
10.12122/j.issn.1673-4254.2020.04.12
- Author:
Xuexia JI
1
;
Yuanbo GUO
1
;
Qianqi QIU
2
;
Zhipeng WANG
1
;
Yan WANG
3
;
Jinquan JI
1
;
Qiang SUN
1
;
Yujing CAI
1
;
Guobin ZHOU
1
Author Information
1. Department of Anesthesiology, Guangdong Provincial People's Hospital/Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
2. Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou 510623, China.
3. Department of Science and Education, Guangdong Provincial People's Hospital/Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
- Publication Type:Journal Article
- Keywords:
endotoxemia;
macrophages;
propofol;
pyrotopsis
- MeSH:
Animals;
Caspase 1;
Lipopolysaccharides;
Macrophages;
Mice;
Propofol;
Pyroptosis
- From:
Journal of Southern Medical University
2020;40(4):525-530
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the molecular mechanism underlying the inhibitory effect of propofol on pyroptosis of macrophages.
METHODS:Macrophages derived from bone marrow were extracted and divided into three groups: control group, LPS+ATP group and propofol+LPS+ATP group. The control group was not given any treatment; LPS+ATP group was given LPS 1 μg/mL stimulation for 4 h, then ATP 4 mM stimulation for 1 h; Propofol+LPS+ATP group was given propofol+LPS 1 μg/mL stimulation for 4 h, then ATP stimulation for 1 h. After treatment, the supernatant and cells of cell culture were collected. the cell activity was detected by CCK8 and flow cytometry. The inflammatory cytokines IL-1βand IL-18 were detected by Elisa. Western blot was used to detect the expression of caspase-1 protein and TLR4 on cell membran Immunohistochemical fluorescence was used to detect apoptosis of cells.
RESULTS:LPS+ATP significantly decreased the viability of the macrophages and increased the cellular production of IL-1β and IL-18, activation of caspase-1 protein and the expression of TLR-4 on the cell membrane ( < 0.05). Treatment with propofol obviously reversed the changes induced by LPS+ATP.
CONCLUSIONS:LPS+ATP can induce pyroptosis of mouse bone marrow-derived macrophages, and propofol effectively inhibits such cell death, suggesting that propofol anesthesia is beneficial during operation and helps to regulate the immune function of in patients with sepsis.
