MiR-204 inhibits invasion and metastasis of breast cancer cells by targeted regulation of HNRNPA2B1.
10.12122/j.issn.1673-4254.2020.06.15
- Author:
Liping ZHANG
1
;
Jun BAI
1
;
Yaqiong HU
1
;
Dandan ZHOU
1
;
Quan ZHENG
1
;
Chonggao YIN
2
;
Qingjie MU
3
;
Hongli LI
4
Author Information
1. Department of Pathology, Weifang Medical University, Weifang 261053, China.
2. College of Nursing, Weifang Medical University, Weifang 261053, China.
3. Clinical Medical College, Weifang Medical University, Weifang 261053, China.
4. Medicine Research Center, Weifang Medical University, Weifang 261053, China.
- Publication Type:Journal Article
- Keywords:
HNRNPA2B1;
breast cancer;
miR-204;
tumor migration and invasion
- MeSH:
Breast Neoplasms;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Gene Expression Regulation, Neoplastic;
Humans;
MicroRNAs;
genetics;
Neoplasm Invasiveness;
Neoplasm Metastasis
- From:
Journal of Southern Medical University
2020;40(6):869-875
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of miR-204 on the invasion and metastasis of breast cancer by targeted regulation of HNRNPA2B1.
METHODS:The bioinformatics database was used to obtain data of the expressions of miR-204 in breast cancer patients and the survival rate of the patients. RT-qPCR was used to detect the expression of miR-204 in breast cancer cell lines. The expression vector GV369-miR-204 was used to overexpress miR-204 in MDA-MB-231 cells. Transwell assay was performed to detect the effect of miR-204 on the migration and invasion ability of the breast cancer cells. The key genes (hub genes) of miR-204 were determined by bioinformatics method. A dual luciferase assay was used to analyze the targeting relationship between miR-204 and HNRNPA2B1. The expression of HNRNPA2B1 in MDA-MB-231 cells after miR-204 overexpression was detected by Western blotting, and Transwell assay was used to examine the changes in the cell invasion ability.
RESULTS:The expression of miR-204 was decreased in both breast cancer tissues, and was significantly lower in breast cancer MDA-MB-231 cells than in MCF-10A cells ( < 0.05). The decreased expression of miR-204 was associated with poorer prognosis of breast cancer patients ( < 0.05). Upregulation of miR-204 in MDA-MB-231 cells significantly inhibited the invasion and migration of the cells ( < 0.05). Analysis of the data from the Starbase revealed that the expression of miR-204-5p was negatively correlated with the expression of HNRNPA2B1, and the expression of HNRNPA2B1 was increased in breast cancer patients ( < 0.05) in association with a poorer prognosis of the patients ( < 0.05). Dual luciferase assay demonstrated that miR-204 could bind to HNRNPA2B1 in a target-specific manner. Western blotting and Transwell assay showed that miR-204 significant inhibited the migration and invasion ability of breast cancer cells by targeting HNRNPA2B1 ( < 0.05).
CONCLUSIONS:miR-204 expression is decreased in breast cancer tissues and cells, and its overexpression can inhibit the invasion and metastasis of breast cancer cells by targeted regulation of HNRNPA2B1.
