Long-chain non-coding RNA MALAT1 regulates paclitaxel resistance of breast cancer cells by targeting miR-485-3p.
10.12122/j.issn.1673-4254.2020.05.13
- Author:
Shatar AINI
1
;
Huanying YAN
2
;
Wei DING
1
;
Lijiang ADI
1
;
Pengcheng SU
1
Author Information
1. Department of Breast and Thyroid, Northen Branch of Xinjiang Uygur Autonomous Region People's Hospital, Urumqi 830000, China.
2. Department of Nursing, Northen Branch of Xinjiang Uygur Autonomous Region People's Hospital, Urumqi 830000, China.
- Publication Type:Journal Article
- Keywords:
MALAT1;
breast cancer;
miR-485-3p;
paclitaxel resistance
- MeSH:
Cell Line, Tumor;
Humans;
MicroRNAs;
Paclitaxel;
RNA, Long Noncoding;
genetics
- From:
Journal of Southern Medical University
2020;40(5):698-702
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the role of long-chain non-coding RNA MALAT1 in modulating paclitaxel resistance in breast cancer cells.
METHODS:Breast cancer SK-BR-3 cells were treated with gradient concentrations of paclitaxel to induce paclitaxel resistance of the cells. The resistant cells were transfected with si-NC, si-MALAT1, pcDNA, pcDNA-MALAT1, miRNC, miR-485-3p mimics, si-MALAT1+anti-miR-NC, or si-MALAT1+anti-miR-485-3p liposomes. Following the transfections, the cells were examined for changes in IC of paclitaxel using MTT assay; the protein expression of P-gp, Bcl-2 and Bax were detected with Western blotting, and a dual luciferase reporter assay was used to detect the binding of MALAT1 to miR-485-3p.
RESULTS:Compared with paclitaxel-sensitive SK-BR-3 cells, paclitaxel-resistant SK-BR-3 cells showed significantly increased the IC of paclitaxel with up-regulated MALAT1 expression and down-regulated miR-485-3p expression ( < 0.05). Silencing MALAT1 or overexpressing miR-485-3p obviously lowered the IC of paclitaxel and the expression of P-gp and Bcl-2 and increased the expression of Bax in SK-BR-3/PR cells ( < 0.05). miR-485-3p was identified as the target of MALAT1, and inhibiting miR-485-3p significantly reverse the effect of MALAT1 silencing on IC of paclitaxel and the expressions of P-gp, Bcl-2 and Bax in SK-BR-3/PR cells ( < 0.05).
CONCLUSIONS:MALAT1 can modulate paclitaxel resistance in breast cancer cells possibly by targeting miR-485-3p to down-regulate P-gp and Bcl-2 and up-regulate Bax.
