Real-time toxicity prediction of stewing system using extractive electrospray ionization mass spectrometry.
10.1016/j.apsb.2019.08.012
- Author:
Zi-Dong QIU
1
;
Jin-Long CHEN
2
;
Wen ZENG
2
;
Ying MA
2
;
Tong CHEN
2
;
Jin-Fu TANG
2
;
Chang-Jiang-Sheng LAI
2
;
Lu-Qi HUANG
1
Author Information
1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, State Key Laboratory Breeding Base of Dao-di Herbs, Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Aconitine;
Aconitum–meat stewing system;
Data mining;
Real-time extractive electrospray ionization mass spectrometry;
Toxic alkaloids;
Toxicity prediction
- From:
Acta Pharmaceutica Sinica B
2020;10(5):903-912
- CountryChina
- Language:English
-
Abstract:
Due to numerous obstacles such as complex matrices, real-time monitoring of complex reaction systems (, medicinal herb stewing system) has always been a challenge though great values for safe and rational use of drugs. Herein, facilitated by the potential ability on the tolerance of complex matrices of extractive electrospray ionization mass spectrometry, a device was established to realize continuous sampling and real-time quantitative analysis of herb stewing system for the first time. A complete analytical strategy, including data acquisition, data mining, and data evaluation was proposed and implemented with overcoming the usual difficulties in real-time mass spectrometry quantification. The complex Fuzi (the lateral root of )-meat stewing systems were real-timely monitored in 150 min by qualitative and quantitative analysis of the nine key alkaloids accurately. The results showed that the strategy worked perfectly and the toxicity of the systems were evaluated and predicated accordingly. Stewing with trotters effectively accelerated the detoxification of Fuzi soup and reduced the overall toxicity to 68%, which was recommended to be used practically for treating rheumatic arthritis and enhancing immunity. The established strategy was versatile, simple, and accurate, which would have a wide application prospect in real-time analysis and evaluation of various complex reaction systems.
