Expression of CREB in children with recurrent wheezing and its effect on ORMDL3 gene expression.
- Author:
Rui JIN
1
;
Zhen-Xing ZHANG
;
Liang-Hua ZHU
;
Li-Li ZHUANG
;
Xiao-Qing CHEN
Author Information
1. Department of Pediatrics, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China. chenxq2002@foxmail.com.
- Publication Type:Journal Article
- MeSH:
Child, Preschool;
Cyclic AMP Response Element-Binding Protein;
Epithelial Cells;
Humans;
Membrane Proteins;
genetics;
Promoter Regions, Genetic;
Respiratory Sounds
- From:
Chinese Journal of Contemporary Pediatrics
2020;22(9):980-983
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression level of cAMP response element-binding protein (CREB) in children with recurrent wheezing under three years of age and its effect on the expression of the serum orosomucoid 1-like protein 3 (ORMDL3) gene.
METHODS:Thirty-six children with recurrent wheezing under three years of age who visited the hospital from June 2017 to June 2019 were selected as the recurrent wheezing group. Twenty-four healthy children from physical examination were selected as the control group. The CREB expression level in peripheral blood was measured by quantitative real-time PCR. Human bronchial epithelial cells (BEAS-2B) were cultured, and dual-luciferase reporter assay and quantitative real-time PCR were used to investigate the effects of overexpression and siRNA interference of CREB on the promoter activity and mRNA expression of the ORMDL3 gene in the BEAS-2B cells.
RESULTS:The expression level of CREB in the recurrent wheezing group was significantly higher than that in the control group (P<0.001). In BEAS-2B cells, overexpression of CREB significantly up-regulated the promoter activity and mRNA expression of the ORMDL3 gene (P<0.05), while siRNA interference of CREB significantly reduced the promoter activity and mRNA expression of the ORMDL3 gene (P<0.05).
CONCLUSIONS:The expression of CREB is increased in children with recurrent wheezing, and CREB may be involved in the pathogenesis of recurrent wheezing by regulating expression of the ORMDL3 gene.
