LC-MS/MS analysis of pharmacokinetics of 3, 29-dibenzoyl karounitriol in rats following administration of Trichosanthes sustained-release pellets.
- Author:
Yingying BIAN
1
;
Chuncai ZOU
1
;
Haiyan YAN
1
;
Dekao SUN
1
;
Qian HU
1
Author Information
1. School Pharmacy, Wannan Medical College, Wuhu 241002, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Area Under Curve;
Benzoates;
pharmacokinetics;
Chromatography, Liquid;
Delayed-Action Preparations;
Male;
Rats;
Rats, Sprague-Dawley;
Reproducibility of Results;
Tandem Mass Spectrometry;
Trichosanthes;
chemistry
- From:
Journal of Zhejiang University. Medical sciences
2020;40(7):995-1000
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the plasma concentration and pharmacokinetics of 3, 29-Dibenzoyl Karounitriol (3, 29-DK) from sustained- release pellets and extracts of Trichosanthes at different time points in rats using high-performance liquid chromatography- tandem mass spectrometry (LC-MS/MS).
METHODS:Healthy male SD rats were given a single gavage of Trichosanthes sustained-release pellets or Trichosanthes extract, and orbital blood samples were taken at different time points within 48 h after drug administration in the pellet group and within 5 h in Trichosanthes extract group for determination of the plasma concentrations of 3, 29-DK using LC-MS/MS. The standard curve of 3, 29-DK content was established, and the specificity, minimum detection limit, precision and accuracy, extraction recovery, stability and matrix effect of LC-MS/MS analysis were assessed. The mean plasms levels of 3, 29-DK at different time points after the drug administration were determined and its pharmacokinetic parameters were calculated using Das 2.0 software.
RESULTS:LC-MS/MS analysis showed a good linearity of 3, 29-DK concentration within the range of 0.5-32 ng/mL, and the results of methodological validation confirmed the validity of this method for biological sample determination. Trichosanthes sustained-release pellets and Trichosanthes extract showed significant differences in their AUC, AUC, MRT, MRT, t and T of 3, 29-DK after administration in rats ( < 0.05).
CONCLUSIONS:Trichosanthes sustained-release pellets are capable of sustained-release of 3, 29-DK in rats, and thus provides a basis for the study of new dosage forms of Trichosanthes.
