Study on chemical constituents of Dendrobium huoshanense stems and their anti-inflammatory activity.
10.19540/j.cnki.cjcmm.20200423.201
- Author:
Shuang-Qiao SU
1
;
Hui JIANG
1
;
Qiang-Ming LI
1
;
Xue-Qiang ZHA
1
;
Jian-Ping LUO
1
Author Information
1. School of Food and Biological Engineering, Hefei University of Technology Hefei 230009, China.
- Publication Type:Journal Article
- Keywords:
Dendrobium huoshanense;
anti-inflammation;
bibenzyl;
chemical constituents
- MeSH:
Anti-Inflammatory Agents;
therapeutic use;
Dendrobium;
Humans;
Inflammation;
drug therapy;
Lipopolysaccharides;
Macrophages;
NF-kappa B;
Nitric Oxide;
Nitric Oxide Synthase Type II
- From:
China Journal of Chinese Materia Medica
2020;45(14):3452-3458
- CountryChina
- Language:Chinese
-
Abstract:
Three bibenzyls 1-3 and six other compounds 4-9 were firstly isolated from Dendrobium huoshanense stems. They were identified as 3',4-dihydroxy-3,5'-dimethoxybibenzyl(1), batatasin Ⅲ(2), 3,4'-dihydroxy-5-methoxy bibenzyl(3), dihydroconiferyl dihydro-p-coumarate(4), syringaresinol(5), 3-(4-hydroxyphenyl)-propionic acid ethyl ester(6),(3-ethylphenyl)-1,2-ethanediol(7),(S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one(8) and loliolide(9). Anti-inflammation assay showed that bibenzyls 1-3 could significantly inhibit the production of nitric oxide(NO) and the expression of tumor necrosis factor α(TNF-α) and interleukin 1β(IL-1β) mRNA in LPS-induced RAW264.7 macrophages. Mechanism study exhibited that the phosphorylation of nuclear factor kappa B(NF-κB) p65, inhibitor of κB(IκB), extracellular regulatedprotein kinase(ERK), c-Jun N-terminalkinase(JNK), p38 and Akt of LPS-induced RAW264.7 macrophages could be remarkably reduced by 1. These results suggested that the inflammatory response of LPS-induced RAW264.7 macrophages could be significantly inhibited by 1-3. Additionally, the anti-inflammatory effect of 1 might be contributed to its ability on the regulation of NF-κB, MAPKs and Akt signaling pathways.
