Comparative study on differences of resin-containing drugs in Dracaena from different appearance based on HS-GC-MS and chemometrics.
10.19540/j.cnki.cjcmm.20200424.201
- Author:
Jing SU
1
;
Yi-Hang LI
1
;
Ling-Juan ZHOU
2
;
Tian-Dao QIN
3
;
Shi-Fang LIU
1
;
Xi CHEN
1
;
Guang LI
1
;
Jin-Yuan MA
3
Author Information
1. Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Jinghong 666100, China Key Laboratory of Dai and Southern Medicine of Xishuangbanna Dai Autonomous Prefecture Jinghong 666100, China.
2. Xishuangbanna Dai Autonomous Prefecture People's Hospital Jinghong 666100, China.
3. College of Tropical Crops, Yunnan Agricultural University Puer 665099, China.
- Publication Type:Journal Article
- Keywords:
HS-GC-MS;
chemometrics;
dragon′s blood;
resin-containing drugs in Dracaena
- MeSH:
Cluster Analysis;
Discriminant Analysis;
Dracaena;
Gas Chromatography-Mass Spectrometry;
Resins, Plant
- From:
China Journal of Chinese Materia Medica
2020;45(14):3467-3474
- CountryChina
- Language:Chinese
-
Abstract:
Resin-containing drugs in Dracaena from four different appearances were analyzed by headspace sampling-gas chromatography-mass spectrometry(HS-GC-MS) metabolomics technique and hierarchical clustering analysis(HCA) chemometrics method. This study was to analyze differential volatile components in resin-containing drugs in Dracaena from different appearance and metabolic pathways. The results of partial least squares discriminant analysis(PLS-DA) and HCA analysis indicated that there was little difference in volatile components between fiber-rich sample and hollow cork cambium sample, however, the volatile components in the two samples compared with whole body resin-containing sample and resin-secreting aggregated sample had a large metabolic difference. Twenty differential metabolites were screened by VIP and P values of PLS-DA. The content of these differential metabolites was significantly higher in whole body resin-containing sample and resin-secreting aggregated sample than in fiber-rich sample and hollow cork cambium sample. Sixteen significant metabolic pathways were obtained through enrichment analysis(P<0.05), mainly involved in terpenoids biosynthesis and phenylpropanoid metabolism. This result provided a reference for further study of resin formation mechanism of resin-containing drugs in Dracaena from different appearances. At the same time, it also provided a reference for establishing a multi-index quality evaluation system.
