Analysis of bacterial community structure and diversity during mountain-agarwood formation.
10.19540/j.cnki.cjcmm.20200527.106
- Author:
Juan LIU
1
;
Jia-Qi GAO
2
;
Su-Yi-le CHEN
3
;
Chao JIANG
1
;
Yuan YUAN
1
;
Shun-Gang JIAO
4
;
Hu-Biao MENG
1
;
Xing-Yun CHAI
4
;
Lu-Qi HUANG
1
Author Information
1. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China School of Pharmacy, Jiangsu University Zhenjiang 212013, China.
3. Alashan League Mongolian Medicine Hospital Alashan League 750300, China.
4. Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
bacterium;
mountain-agarwood;
population diversity;
population structure
- MeSH:
Bacteria;
genetics;
China;
DNA, Ribosomal;
Resins, Plant;
Thymelaeaceae
- From:
China Journal of Chinese Materia Medica
2020;45(15):3651-3658
- CountryChina
- Language:Chinese
-
Abstract:
As an important substitute for agarwood, mountain-agarwood, belonging to the family Oleaceae, comes from the root, stem and thick branch of Syringa pinnatifolia, which has a wide range of application in Inner Mongolia, China. It has good clinical efficacy in the use of cardiovascular diseases. However, the formation speed of mountain-agarwood is extremely slow, and its cultivated seedlings have low resin content. Therefore, how to speed up the formation of mountain-agarwood and increase the resin content is a hot research topic in this field. In this work, 16 S rDNA amplicon sequencing method was used to systematically analyze the bacterial communities of different samples of mountain-agarwood. Our data revealed that the samples of mountain-agarwood had more obvious species diversity than the ones of non-mountain-agarwood, especially the wild mountain-agarwood samples. By analysis of bacterial community composition and species abundance, Sphingomonas, Modestobacter and unidentified Cyanobacteria genus were three dominant bacterial genera in all samples. In addition, there are two identified genera of dominant bacteria, namely Actinoplanes and Microbacterium in both wild and cultivated mountain-agarwood, by bacterial community composition and species richness analysis. Meanwhile, Roseomonas was the dominant bacterial genus in both wild and cultivated non-mountain-agarwood samples. Our work could provides basic data for exploring the mechanism of the mountain-agarwood formation, and help to exploit resource of endophytic bacteria reasonably.
