Effect of Huaier aqueous extract on growth and metastasis of human non-small cell lung cancer NCI-H1299 cells and its underlying mechanisms.
10.19540/j.cnki.cjcmm.20200310.401
- Author:
Ying-Ying TIAN
1
;
Ai-Lin YANG
1
;
Xiao-Nan CHEN
1
;
Hao-Miao REN
1
;
Ya-Xin LIU
1
;
Hai-Ling QIU
1
;
Lei-Meng-Yuan TANG
1
;
Hui-Ming HUANG
1
;
Peng-Fei TU
1
;
Zhong-Dong HU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
Huaier aqueous extract;
apoptosis;
ferroptosis;
human non-small cell lung cancer cells;
metastasis;
proliferation
- MeSH:
Apoptosis;
Carcinoma, Non-Small-Cell Lung;
Cell Line, Tumor;
Cell Proliferation;
Complex Mixtures;
Humans;
Lung Neoplasms;
Trametes
- From:
China Journal of Chinese Materia Medica
2020;45(15):3700-3706
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the effect of Huaier aqueous extract on the growth and metastasis of human non-small cell lung cancer NCI-H1299 cells and its underlying mechanisms. MTT assay was used to detect the effect of Huaier aqueous extract on the proliferation of NCI-H1299 cells. Flow cytometry was used to examine the effect of Huaier aqueous extract on the apoptosis, cell cycle, and ROS level of NCI-H1299 cells. Wound healing assay was used to evaluate the effect of Huaier aqueous extract on the migration ability of NCI-H1299 cells. Western blot was used to detect the levels of proteins involving apoptosis, epithelial-mesenchymal transition(EMT), and MAPK signaling pathway in NCI-H1299 cells exposed to Huaier aqueous extract. The results showed that Huaier aqueous extract inhibited the proliferation of NCI-H1299 cells, and induced cell-cycle arrest at the phase S. Huaier aqueous extract promoted the apoptosis of NCI-H1299 cells by down-regulating the expression of anti-apoptotic protein Bcl-2. Moreover, Huaier aqueous extract increased ROS level and induced ferroptosis in NCI-H1299 cells. EMT played a critical role in cancer metastasis. Huaier aqueous extract reduced the migration ability of NCI-H1299 cells by inhibiting EMT of NCI-H1299 cells. In addition, this study revealed that Huaier aqueous extract inhibited MAPK signaling pathway in human non-small cell lung cancer NCI-H1299 cells, which may be one of Huaier's mechanisms in inhibiting growth and metastasis of NCI-H1299 cells. This study provides a new theoretical basis for the clinical treatment of lung cancer with Huaier, and important reference significance for further studies on the anti-tumor mechanisms of Huaier.
