Recent advances in biosynthesis of forskolin.
10.19540/j.cnki.cjcmm.20200512.201
- Author:
Hui-Ren YUAN
1
;
Ming-Gang LI
1
;
Jiang-Yuan ZHAO
1
;
Meng-Liang WEN
1
;
Xiu-Lin HAN
1
Author Information
1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, National Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Institute of Microbiology, School of Life Sciences,Yunnan University Kunming 650091, China.
- Publication Type:Journal Article
- Keywords:
Coleus forskohlii;
cytochrome P450 enzymes;
forskolin;
synthetic biology
- MeSH:
Biosynthetic Pathways;
Colforsin
- From:
China Journal of Chinese Materia Medica
2020;45(16):3790-3796
- CountryChina
- Language:Chinese
-
Abstract:
Forskolin is a complex labdane plant diterpenoid, which has been used in the treatment of a variety of diseases based on its activity as an activator of adenosine monophosphate(cAMP) cyclase. Natural forskolin exists only in the cork layer of the root of Coleus forskohlii. Due to the complexity of the extraction and chemical synthesis processes, the yield and purity of forskolin cannot meet commercial requirements. In recent years, with the rapid development of synthetic biology and the analysis and interpretation of many diterpene biosynthetic pathways, a new approach has been provided for the green production of forskolin. In this paper, the structure, activity, biosynthetic pathway and the heterologous biosynthesis of forskolin were reviewed. The problems and solutions in the heterologous biosynthesis of forskolin were also discussed and summarized, which will provide references for the construction of high-yielding forskolin engineering strains.
