Transcriptome analysis of seed embryo in dormancy and dormancy release state of Thesium chinense.
10.19540/j.cnki.cjcmm.20200506.109
- Author:
Cheng-Cai ZHANG
1
;
Zhen GAO
1
;
Li-Na LUO
1
;
Zi-Xuan ZHANG
1
;
Jiang WANG
1
;
Fu-Hua LU
2
;
Zeng-Xu XIANG
1
Author Information
1. College of Horticulture, Nanjing Agricultural University Nanjing 210095, China.
2. Institute of Medicinal Plant Development,Peking Union Medical College, Chinese Academy of Medical Sciences Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
Thesium chinense;
differential genes;
dormancy;
exogenous GA_3;
transcriptome
- MeSH:
Gene Expression Profiling;
Gene Expression Regulation, Plant;
Plant Dormancy;
Plant Growth Regulators;
Santalaceae;
Seeds;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2020;45(16):3837-3843
- CountryChina
- Language:Chinese
-
Abstract:
We used exogenous GA_3 to break the seed dormancy of Thesium chinense. We used high-throughput sequencing technology was used to sequence the transcriptome of dormant seed embryos and dormancy breaking seed embryos of Th. chinense, and the data was analyzed bioinformatically and systematically. The results showed that exogenous GA_3 could effectively break the seed dormancy of Th. chinense; 73 794 up-regulated genes and 42 776 down regulated genes were obtained by transcriptome sequencing; 116 570 diffe-rential genes were annotated by GO function to GO items such as metabolism process, cell process, cell, cell component, binding and catalytic activity. A total of 133 metabolic pathways were found by Pathway analysis of 26 508 differentially expressed genes. In the process of dormancy release, DEGs were mainly enriched in translation, carbohydrate metabolism, folding, classification, degradation and amino acid metabolism. Based on the annotation results in KEGG database, 20 metabolic pathways related to dormancy release were found. Dormancy release of Th. chinense seeds is a complex biological process, including cell morphology construction, secondary metabolite synthesis, sugar metabolism and plant signal transduction, among which plant hormone signal transduction is one of the key factors to regulate dormancy release. The results of qRT-PCR showed that the sequencing results were consistent with the actual results.
