Proteomics and peptidomics analysis of Sepiae Endoconcha.
10.19540/j.cnki.cjcmm.20200508.201
- Author:
Rui LIU
1
;
Shuang WEI
1
;
Xin-Zhi WANG
1
;
Jian-Ming CHENG
1
;
Hao WU
1
Author Information
1. Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics Nanjing 210023, China Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
Sepiae Endoconcha(cuttlebone);
marine traditional Chinese medicine;
material basis;
peptide;
protein
- MeSH:
Cations;
Databases, Protein;
Mass Spectrometry;
Peptides;
Proteomics
- From:
China Journal of Chinese Materia Medica
2020;45(16):3883-3889
- CountryChina
- Language:Chinese
-
Abstract:
Shotgun based proteomics and peptidomics analysis were used to investigate the proteins and peptides in marine traditional Chinese medicine(TCM) Sepiae Endoconcha(cuttlebone). Peptides were extracted from cuttlebone by acidified methanol, and then strong cation exchange(SCX) resin was used to enrich those peptides. Also, proteins from cuttlebone were extracted and digested by trypsin. nano-LC Q Exactive Orbitrap mass spectrometry was used to analyze proteins and peptides from cuttlebone. As a result, a total of 16 proteins and 168 peptides were identified by protein database search, and 328 peptides were identified by De novo sequencing. The identified proteins were hemocyanin, enolase, myosin, actin, calmodulin, etc., and the identified peptides were derived from actin, histone, and tubulin. All these proteins and peptides were important components in cuttlebone, which would provide important theoretical and research basis for marine TCM cuttlebone investigations.
