Simultaneous determination and analysis of amino acids and nucleosides in Dendrobium by UHPLC-QTRAP-MS/MS.
10.19540/j.cnki.cjcmm.20200513.201
- Author:
Jun-Lin LIU
1
;
Nian-Jun YU
2
;
Li-Hua XING
1
;
Rui WANG
3
;
Jun XU
1
;
Dai-Yin PENG
2
;
Xiao-Hui TONG
3
;
Jin-Mei OU
3
;
Xiang-Lin HE
4
Author Information
1. Anhui University of Chinese Medicine Hefei 230012, China Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine Hefei 230012, China.
2. Anhui University of Chinese Medicine Hefei 230012, China Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine Hefei 230012, China Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement Hefei 230012, China Anhui Province Key Laboratory of Chinese Medicinal Formula Hefei 230012, China.
3. Anhui University of Chinese Medicine Hefei 230012, China.
4. Huoshan Changchong Chinese Medicinal Material Development Co., Ltd. Huoshan 237200, China.
- Publication Type:Journal Article
- Keywords:
Dendrobium;
UHPLC-QTRAP-MS/MS;
amino acids;
content determination;
nucleoside
- MeSH:
Amino Acids;
Chromatography, High Pressure Liquid;
Dendrobium;
Nucleosides;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2020;45(16):3890-3899
- CountryChina
- Language:Chinese
-
Abstract:
By using multivariate statistical analysis to evaluate essential quality, and provide scientific basis for their comprehensive utilization, we established an UHPLC-QTRAP-MS/MS method for the fast, precise, efficient determination of 21 kinds of amino acids and 10 kinds of nucleosides in different species of Dendrobium. The analysis was performed on a Waters XBridge Amide column(2.1 mm×100 mm,3.5 μm) with elution by mobile phase of 0.2% formic acid in water-0.2% formic acid in acetonitrile at a flow rate of 0.2 mL·min~(-1) with the column temperature at 30 ℃. The target compounds were analyzed by the positive ion multiple reaction monitoring(MRM) mode. The comprehensive evaluation of different species of Dendrobium was carried out by PCA and TOPSIS analysis. All 21 kinds of amino acids and 10 nucleosides showed good linearity among certain concentration range(r>0.999), the RSDs of the stability, precision, and repeatability tests were less than 3.0%. The recovery rate was in the range from 93.31% to 107.5%, and RSD was in the range of 1.1%-3.7%. The comprehensive evaluation index obtained with PCA showed that D. huoshanense was significantly higher than others regarding amino acids and D. officinale has higher nucleosides than other species. The biggest C_i difference of TOPSIS was 68.7%, and comprehensive evaluation showed that D. huoshanense produced the highest comprehensive quality. The method is precise, fast and efficient and can provide reliable basis for further researches and intrinsic quality control of Dendrobium.
