Effect and mechanism of Bidens pilosa decoction on non-alcoholic fatty liver induced by high fat and high glucose in mice.
10.19540/j.cnki.cjcmm.20200321.401
- Author:
Xiao-le GAO
1
;
Leng-Xin DUAN
1
;
Ke-Ke QIU
1
;
Man-Lin GUO
1
;
Ye-Lin JIAO
1
;
Dong-Mei WANG
1
Author Information
1. Medical School, Henan University of Science and Technology Luoyang 471023, China.
- Publication Type:Journal Article
- Keywords:
Bidens pilosa decoction;
endoplasmic reticulum stress(ERS);
high fat and high glucose;
metformin;
non-alcoholic fatty liver(NAFLD)
- MeSH:
Animals;
Apoptosis;
Bidens;
Endoplasmic Reticulum Stress;
Endoribonucleases;
Glucose;
Mice;
Non-alcoholic Fatty Liver Disease;
Protein-Serine-Threonine Kinases
- From:
China Journal of Chinese Materia Medica
2020;45(16):3915-3921
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate the effect and possible mechanism of Bidens pilosa decoction on non-alcoholic fatty liver disease(NAFLD) induced by high fat and high glucose in mice. Bald/c mice were randomly divided into normal group, model group, metformin(200 mg·kg~(-1)) treatment group, Bidens pilosa decoction(10 g·kg~(-1)) treatment group, metformin and B. pilosa decoction(100 mg·kg~(-1)+5 g·kg~(-1)) treatment group. Except for the normal group, mice in the other four groups were fed with high-fat and high-glucose diet for 8 weeks to establish the non-alcoholic fatty liver model. After 4 weeks of treatment, blood was collected from the eyeballs, the mice were sacrificed, and relevant indicators were detected. The results showed that compared with the model group, blood lipid and blood glucose levels of each treatment group were significantly lower(P<0.05); HE staining results showed that liver pathological damage in each treatment group was significantly improved; oil red O staining results showed fat distribution in each treatment group significantly reduced(P<0.01); immunohistochemical staining showed that glucose regulated the protein expression of protein 78(GRP78) in liver tissues of each treatment group was also significantly reduced(P<0.01); Western blot results showed that endoplasmic reticulum stress signal pathway-related factors GRP78, phosphorylated-protein kinase R-like ER kinase(p-PERK), eukaryotic translation-initiation factor 2α(eIF2α), activating transcription factor 4(ATF4), C/EBP homologous protein(Chop), inositol requiring 1α(IRE1α), and cleaved-cysteinyl aspartate specific proteinase 12(cleaved-caspase-12) were significantly reduced(P<0.01). The results of the combined drug treatment group were better than those of the single drug treatment group. These results showed that B. pilosa decoction had the effect in improving non-alcoholic fatty liver, and its mechanism may be related to the down-regulation of the expression of endoplasmic reticulum stress(ERS)-related factors, and the reduction of the apoptosis of hepatocytes caused by ERS and the down-regulation of blood lipid and blood glucose levels.
